灰旱獭-长尾黄鼠鼠疫疫源地监测中应用ELISA检测F1抗体的可行性分析

Feasibility of enzyme-linked immunosorbent assay in detection of plague F1 antibody in Marmota baibacina-Citellus undulatus plague focus

目的观测灰旱獭-长尾黄鼠鼠疫疫源地监测中应用酶联免疫吸附试验夹心法(ELISA)检测鼠疫F1抗体的效果,分析应用前景。方法 ELISA和间接血凝试验(IHA)同步检测动物血清鼠疫F1抗体,比较两种方法的阳性率、反应滴度。结果 ELISA和IHA检测血清的鼠疫F1抗体,长尾黄鼠阳性率分别为11.71%(118/1 008)和1.73%(21/1 216),平均滴度分别为27.805和21.254;牧犬阳性率分别为38.89%(28/72)和10.71%(7/75),平均滴度分别为27.731和21.50。ELISA检测长尾黄鼠、牧犬血清鼠疫F1抗体的阳性率和平均滴度均高于IHA,差异非常显著(P<0.001)。结论两种方法同步用于鼠疫监测,可及时发现并纠正单一方法因试剂变质或失效出现的错误结果,为制定防控措施提供准确的监测资料。

The effect of enzyme-linked immunosorbent assay （ELISA） in detecting plague F1 antibody was investigated and its application perspective was analyzed in Marmota baibacina-Citellus undulatus plague focus. Plague F1 antibody was de- tected synchronously with ELISA and IHA （indirect hemagglutination test） in animal serum, and the positive rate and reaction titer were compared. The positive rates of Citellus undulatus were 11. 71% （118/1 008） and 1. 73% （21/1 216） for ELISA and IHA, and the average titers were 27.805 and 21.234 , respectively. The positive rates of husbandry dogs were 38.89%（28/ 72） and 10.71% （7/75） for ELISA and IHA, and the average titers were 27.732 and 21.50 , respectively. ELISA had higher posi- tive rate and average titer than IHA in detecting plague F1 antibody of animal serum （P（0. 001）. The error result of a single method due to reagent deterioration or failure could be timely discovered and corrected by synchronously using the two methods in plague surveillance, and exact monitoring data could be provided for the development of prevention and control.