低浓度硫化氢对类风湿性关节病变的软骨保护

Protective effects of low-concentration hydrogen sulfide on rheumatoid joint disease

背景:类风湿性关节炎是一种常见的慢性系统性自身免疫疾病,以关节滑膜病变、骨质侵蚀为主要特征,临床上的治疗仍然比较棘手。目的:探讨外源性硫化氢对类风湿性关节炎大鼠关节病变的影响。方法:将SD大鼠分为4组:空白组、模型组、高浓度硫化氢干预组、低浓度硫化氢干预组。干预2,4周时,测量关节炎指数,血清炎症因子肿瘤坏死因子α和白细胞介素1β水平,滑膜组织病理学变化,膝关节标本Ⅱ型胶原染色的平均吸光度值。结果与结论:从关节炎指数,血清炎症因子水平以及滑膜染色炎性浸润方面看,模型组以及硫化氢干预组均高于空白组,高浓度硫化氢干预组明显高于模型组(P<0.05),低浓度硫化氢干预组低于模型组(P<0.05)。在膝关节软骨Ⅱ型胶原染色的平均吸光度值方面,模型组以及2个硫化氢干预组均低于空白组,高浓度硫化氢干预组明显低于模型组(P<0.05),低浓度硫化氢干预组高于模型组(P<0.05)。结果可见硫化氢对类风湿关节炎症有调节作用,高浓度的硫化氢加重关节炎症及软骨降解,低浓度的硫化氢减轻关节炎症并对软骨有保护作用。

BACKGROUND：Rheumatoid arthritis is a common chronic systemic autoimmune disease which is characterized by synovial lesions and bone erosion. The clinical treatment is stil a difficulty.OBJECTIVE：To explore the effect of exogenous hydrogen sulfide on the articular lesions in rheumatoid arthritis rats. METHODS：Sprague-Dawley rats were divided into four groups：control group, model group, high concentrations of hydrogen sulfide intervention group, low concentrations of hydrogen sulfide intervention group. At 2 and 4 weeks after intervention, the arthritis index, levels of serum inflammatory cytokines （tumor necrotic factor-αand interleukin-1β）, pathological change of synovial tissue, average absorbance of knee joint specimens using col agen-II immunohistochemical staining were measured. RESULTS AND CONCLUSION：The arthritis index, levels of serum inflammatory cytokines and inflammatory infiltration of synovial staining in the model group and two intervention groups were higher than that in control group, the high concentrations of hydrogen sulfide intervention group was significantly higher than the model group （P＜0.05） while the low concentrations of hydrogen sulfide intervention group was lower than the model＆amp;nbsp;group （P＜0.05）. The average absorbance of knee cartilage specimens using col agen-II immunohistochemical staining was lower in the model group and two hydrogen sulfide intervention groups compared with the control group, the high concentrations of hydrogen sulfide intervention group was significantly lower than the model group （P〈0.05） while low concentrations of hydrogen sulfide intervention group was higher than the model group （P〈0.05）. Hydrogen sulfide has a regulatory effect on rheumatoid arthritis, high concentrations of hydrogen sulfide aggravate arthritis and cartilage degradation, low concentrations of hydrogen sulfide reduce arthritis and protect cartilage.