间歇性完全鼻阻塞诱导幼年大鼠颏舌肌低氧诱导因子1α的表达

Expression of HIF-1α in the genioglossus associated with induced bilateral intermittent nasal obstruction in young rats

目的:研究幼鼠在双侧间歇性鼻阻塞时低氧诱导因子1α(hypoxia inducible factor,HIF-1α)的表达变化,探讨双侧鼻阻塞对生长发育期大鼠颏舌肌的影响。方法:将30只4周龄SD大鼠(Sprague-1Dawley rat,SD rat)分为3组。A组为双侧鼻阻塞组,常氧条件下鼻孔双侧阻塞4 h(8:00 am-12:00 am),分别阻塞21 d和55 d;B组为单侧鼻阻塞组,常氧条件下鼻孔单侧阻塞4 h/d(8:00 am-12:00 am),分别阻塞21 d和55 d;C组为对照组,常氧条件下饲养。取出颏舌肌.分别进行HE染色和免疫组织化学染色,检测颏舌肌中HIF-1α的表达,采用SPSS13.0软件包对数据进行统计学分析。结果:在双侧鼻阻塞组,颏舌肌HIF-1α的表达随鼻阻塞时间的延长而增加,单侧鼻阻塞组以及对照组中颏舌肌均无HIF-1α表达。结论:在双侧鼻阻塞的未成年大鼠中,颏舌肌表达HIF-1α,并且随着鼻阻塞时间的延长,HIF-1α的表达不断增加.

PURPOSE： To investigate the expression of HIF-1a in the genioglossus associated with induced bilateral intermittent nasal obstruction in young rats. METHODS： Thirty 4-week-old SD rats were employed and equally divided into 3 groups. In group A, both nostrils were occluded by nose plugs. In group B, the right nostril was occluded. In group C, no obstruction of the nose was performed as control group. The obstruction time was from 8 am to 12 am everyday, and the period was 21 d and 55 d. The genioglossus was taken for HE, and immunohistochemical staining was used to detect the expression of HIF-1a. The data was analyzed with SPSS 13.0 software package. RESULTS： The rats were sacrificed at the 21th day and 55th day, respectively. The expression of HIF-1a in group A was significantly higher than that in group B and group C, and became stronger with the increasing of obstruction time. CONCLUSIONS： Oral breathing caused by bilateral intermittent nasal obstruction in young rats results in overexpression of HIF-1a in the genioglossus.