微管相关蛋白轻链3和beclin-1在子宫内膜异位症中的表达及意义

Expression of microtubule-associated protein light chain 3 and beclin-1 in endometriosis

目的：研究自噬相关蛋白微管相关蛋白轻链3（LC-3）和beclin-1在子宫内膜异位症患者异位内膜组织中的表达情况,探讨其与临床分期的关系.方法：采用免疫组织化学及免疫印迹检测80例子宫内膜异位症患者（研究组）及20例正常子宫内膜（对照组）组织中LC-3和beclin-1蛋白的定位及定量情况.结果：研究组异位内膜组织中LC-3和beclin-1的表达较对照组显著下调,并且随着r-AFS期别程度的增加,LC-3和beclin-1蛋白表达呈逐渐下降趋势.结论：LC-3和beclin-1在子宫内膜异位症中处于低表达状态,且与子宫内膜异位症的发生发展及严重程度呈负相关.

Objective： To investigate the expression of the autophagy-related protein microtubule-associated protein light chain 3 （LC-3） and beclin-1 in the ectopic endometrial tissues of patients with endometriosis, and to further explore the relation with the pathogenesis of endometriosis. Methods： The localization and quantification of LC-3 and beclin-1 protein was detected in 80 cases of uterine endometriosis patients （study group） and 20 cases of normal endometrium tissues （control group） by using immunohistochemistry and Western blotting analysis. Results ： The expressions of LC-3 and beclin-1 in the ectopic endometrial tissues of the study group were significantly lower compared with the control group, and the levels of r-AFS increased. The expression of LC-3 and beclin-1 protein decreased gradually. Conclusion： A low expression state in endometriosis appears in LC-3 and beclin-1, and has a relation with the occurrence and development of endometriosis.