大鼠大脑中动脉栓塞对脊髓前角运动神经元乙酰胆碱酯酶的影响

Effect of middle cerebral artery occlusion on AChE of anterior horn motor neurons in rat

目的：探讨大鼠大脑中动脉栓塞（MCAO）对脊髓前角运动神经元外侧核群乙酰胆碱酯酶（AChE）的影响.方法：20只雄性SD大鼠随机分为4组,均采用线栓法建立一侧大鼠MCAO致脑缺血模型,术后大鼠分别存活1、2、4、8周,取脊髓颈膨大.采用亚铁氰化铜法显示AChE,Image-Pro Plus 6.0图像分析软件对脊髓前角运动神经元外侧核群AChE反应阳性区域的累积光密度（IOD）进行定量分析.结果：MCAO 1周组实验侧脊髓前角运动神经元外侧核群AChE的IOD值高于对照侧,MCAO 2周组开始低于对照侧,且病程越长此变化更为明显.结论：大鼠MCAO可导致脊髓前角运动神经元外侧核群AChE的含量发生改变.

Objective： To investigate the effects of middle cerebral artery occlusion （MCAO） on AChE in the anterior horn motor neurons later nucleus in rats. Methods： 20 male SD rats were randomly divided into 4 groups, the cerebral ischemia on one side was established by MCAO rat model on the same side, and the rats were allowed to survive for 1 week, 2 weeks, 4 weeks and 8 weeks after operation. The cervical enlargement was taken and displayed by Karnovsky-Roots. Image-Pro Plus 6.0 image analysis system was used to analyze the integrated optical density （IOD） of AChE positive region. Results： The AChE IOD of the anterior horn motor neurons of lateral nucleus in 1 week group on the experimental side was higher than that on the control side. The AChE IOD in 2 weeks group on the experimental side was lower than that on the control side. The changes were more obvious with the extension of the course of disease. Conclusion： MCAO in rats can lead to the diversification of AChE in the anterior horn motor neurons of lateral nucleus.