摘要
目的:探讨心衰患者血清中β1肾上腺素受体自身抗体(β1-AA)对不同亚型T淋巴细胞增殖能力的影响。方法利用IgG亲和柱从β1-AA阳性的心衰患者血清中提纯IgG;利用流式分选技术,分选得到CD3+CD4+以及CD3+CD8+T淋巴细胞;采用CCK-8试剂盒检测β1-AA对不同亚型T淋巴细胞增殖能力的影响;使用AnnexinⅤ/PI试剂盒及乳酸脱氢酶( LDH)水平反映细胞的损伤情况。结果β1-AA可浓度依赖性抑制活化的淋巴细胞增殖促进其损伤的发生,该效应可被β1受体阻断剂所阻断;β1-AA可诱导CD3+CD4+以及CD3+CD8+T淋巴细胞发生凋亡和坏死,并抑制其增殖。结论心衰患者血清中存在的高滴度的β1-AA可通过诱导不同亚型T 淋巴细胞发生损伤而抑制其增殖,提示,β1-AA阳性的血清除了可导致心肌损伤外还存在诱发免疫系统紊乱的危险。
Objective To investigate the effects of β1-adrenergic receptor autoantibodies (β1-AA) on the proliferation of different subtypes of T lymphocytes in patients with heart failure .Methods β1-AA-positive IgG antibodies isolated from patients with heart failure were purified by using affinity chromatog -raphy.CD3 +CD4 +T and CD3 +CD8 +T lymphocytes were sorted by flow cytometry analysis .The prolifera-tion of different subtypes of T lymphocytes was tested by using CCK-8 kit.Tests for lactate dehydrogenase ( LDH) level and cell apoptosis were performed to evaluate T lymphocytes damage .Results The prolifera-tion of activated lymphocytes was inhibited by β1-AA isolated from the serum of patients with heart failure in a concentration dependent manner , but it could be blocked by β1 receptor blocker .The damage of lympho-cytes induced by β1-AA was increased.Moreover, β1-AA promoted the necrosis and apoptosis of CD 3 +CD4 +T and CD3 +CD8 +T lymphocytes and thus inhibited the proliferation of them .Conclusion β1-AA isolated from the serum of patients with heart failure inhibited the proliferation of CD 3 +CD4 +T and CD3 +CD8 +T lymphocytes through increasing the necrosis and apoptosis of them .This study suggests that β1-AA might induce immune disorders in addition to causing pathological changes in heart tissues .
出处
《中华微生物学和免疫学杂志》
CAS
CSCD
北大核心
2014年第3期205-211,共7页
Chinese Journal of Microbiology and Immunology
基金
国家自然科学基金资助课题(81070263,81300180)
2013年度科技创新基地培育与发展工程专项项目(z131107002813041)