心衰患者血清中β1肾上腺素受体自身抗体抑制不同亚型T淋巴细胞的增殖

β1-adrenergic receptor autoantibodies inhibit the proliferation of different subtypes of T lymphocytes in patients with heart failure

目的：探讨心衰患者血清中β1肾上腺素受体自身抗体（β1-AA）对不同亚型T淋巴细胞增殖能力的影响。方法利用IgG亲和柱从β1-AA阳性的心衰患者血清中提纯IgG；利用流式分选技术，分选得到CD3＋CD4＋以及CD3＋CD8＋T淋巴细胞；采用CCK-8试剂盒检测β1-AA对不同亚型T淋巴细胞增殖能力的影响；使用AnnexinⅤ/PI试剂盒及乳酸脱氢酶（ LDH）水平反映细胞的损伤情况。结果β1-AA可浓度依赖性抑制活化的淋巴细胞增殖促进其损伤的发生，该效应可被β1受体阻断剂所阻断；β1-AA可诱导CD3＋CD4＋以及CD3＋CD8＋T淋巴细胞发生凋亡和坏死，并抑制其增殖。结论心衰患者血清中存在的高滴度的β1-AA可通过诱导不同亚型T 淋巴细胞发生损伤而抑制其增殖，提示，β1-AA阳性的血清除了可导致心肌损伤外还存在诱发免疫系统紊乱的危险。

Objective To investigate the effects of β1-adrenergic receptor autoantibodies （β1-AA） on the proliferation of different subtypes of T lymphocytes in patients with heart failure .Methods β1-AA-positive IgG antibodies isolated from patients with heart failure were purified by using affinity chromatog -raphy.CD3 ＋CD4 ＋T and CD3 ＋CD8 ＋T lymphocytes were sorted by flow cytometry analysis .The prolifera-tion of different subtypes of T lymphocytes was tested by using CCK-8 kit.Tests for lactate dehydrogenase （ LDH） level and cell apoptosis were performed to evaluate T lymphocytes damage .Results The prolifera-tion of activated lymphocytes was inhibited by β1-AA isolated from the serum of patients with heart failure in a concentration dependent manner , but it could be blocked by β1 receptor blocker .The damage of lympho-cytes induced by β1-AA was increased.Moreover, β1-AA promoted the necrosis and apoptosis of CD 3 ＋CD4 ＋T and CD3 ＋CD8 ＋T lymphocytes and thus inhibited the proliferation of them .Conclusion β1-AA isolated from the serum of patients with heart failure inhibited the proliferation of CD 3 ＋CD4 ＋T and CD3 ＋CD8 ＋T lymphocytes through increasing the necrosis and apoptosis of them .This study suggests that β1-AA might induce immune disorders in addition to causing pathological changes in heart tissues .