摘要
目的利用各种生物信息学工具预测hsa-miR-1908上游启动子功能,以进一步研究其在人脂肪细胞中的转录调控机制。方法应用Ensemble数据库获取hsa-miR-1908上游启动子序列,利用多种在线相关软件预测出甲基化部位和转录因子结合部位。结果获取hsa-miR-1908上游启动子序列全长1458bp。miR-1908启动子序列中CpG岛位于(438~756)bp、(836937)bp、(979~1374)bp处,CpG岛的存在会抑制miR-1908启动子的转录。miR-1908有15个转录因子的结合位点。结论miRNA启动子相关生物信息学的研究,提高对启动子的研究效率,为进一步研究miR-1908的转录调控机制提供了重要的信息。
Objective To predict the functions of hsa-miR-1908 promoter using various bioinformatic tools, and to provide clues for further study on transcriptional regulation mechanism of miR-1908 in human adipocytes. Methods The promoter se- quence of miR-1908 was obtained from Ensemble, and then the CpG islands and transcription factor binding sites were pre- dicted by a variety of online bioinformatic tools. Results The length of the miR-1908 promoter sequence was 1 458 bp. The CpG islands, which inhibited the transcription of miR-1908, were located at (438-756) bp, (836-937) bp and (979-1374) bp. Meanwhile, 15 transcription factor binding sites were found in the promoter sequence of miR-1908. Conclusions miRNA up- stream promoter related bioinformatics can not only improve the efficiency of microRNA promoter research, but also provide further important information on transcriptional regulation of miR-1908.
出处
《临床儿科杂志》
CAS
CSCD
北大核心
2014年第4期379-383,共5页
Journal of Clinical Pediatrics
基金
国家自然科学基金资助项目(No.81100618)