YB-1蛋白在结肠癌组织中的表达部位及意义

The Location and Significance of Y-box Protein 1 Expressed in Colorectal Cancer Tissues

目的:探索YB-1蛋白在结肠癌组织、正常结肠组织、结肠癌细胞株、正常结肠细胞株中的表达量及其与结肠癌患者临床病理的关系。方法:采用免疫组化检测YB-1蛋白在103例结肠癌组织及相应癌旁组织、30例正常结肠组织中的表达情况;采用Western blot法检测核移位YB-1蛋白在结肠癌组织、相应癌旁组织、正常结肠组织、人结肠腺癌细胞株LS-174T、正常细胞株SW480中的表达。结果:免疫组化结果显示YB-1蛋白主要表达于癌细胞胞浆,其阳性率为(75.73%,78/103),明显高于癌旁组织(37.86%,39/103)及正常组织(30%,9/30),其差异有统计学意义(P<0.05)。其中37例(35.92%,37/103)结肠癌组织伴有明显核阳性表达,且核阳性表达与结肠癌肿瘤直径大小、有无淋巴结转移、有无远处转移或直接侵犯及癌细胞分化程度相关(P<0.05)。Western blot检测同样证实核移位YB-1蛋白在结肠癌组织中(0.382±0.095)明显高于癌旁组织(0.251±0.043)及正常结肠组织(0.238±0.045),其差异有统计学意义(F=21.268,P<0.05)。核移位YB-1蛋白在LS-174T中的表达(0.548±0.031)明显高于SW480(0.269±0.017),其差异有明显统计学意义(F=64.273,P<0.05)。结论:YB-1蛋白量的变化与核转移及结肠癌细胞的增殖分化密切相关,有可能成为结肠癌治疗的新靶点。

Objective To explore the expression quantity of YB-1 protein which expressed in colorectal cancer tissue and normal colon tissue, in colon cancer cell lines and normal colonic cell lines, and research the relationship with clinical pathology in colon cancer patients. Methods We used immunohistochemical method to detect YB-1 protein in 103 cases of colon cancer tissue and corresponding tissue adjacent to carcinoma, and the expression in 30 cases of normal colon tissue. We used Western blot method to detect the quantity of YB-1 protein expressed inside nucleus in colorectal cancer tissues, tissue adjacent to carcinoma, normal colon tissue, LS-174T cell lines and SW480 cell lines. Results Immunohistochemical results showed YB-1 protein expres-sion mainly in cytoplasm, the positive rate in cancer tissues（75.73%,78/103）was more higher than tissue adja-cent to carcinoma（37.86%,39/103） and normal tissue（30%,9/30）. The difference was statistical significance （P〈0.05）. 37 cases（35.92%,37/103）showed that YB-1 protein expressed inside nucleus, and the positive ex-pression inside nucleus was related to the tumor diameter size, lymph node metastasis, distant metastasis or di-rect invasion and the degree of cell differentiation （P〈0.05）. Western blot results also confirmed that YB-1 pro-tein expressed inside nucleus in cancer tissue （0.382±0.095） were significantly higher than the tissue adjacent to carcinoma （0.251±0.043） and normal tissue （0.238±0.045）. The difference was statistical significance （F=21.268, P〈0.05）. And YB-1 protein expressed inside nucleus in LS-174T （0.548 ± 0.031） was significantly higher than sw480 cell lines （0.269 ± 0.017）. The difference was statistical significance （F=64.273 , P〈0.05）. Conclusion The change of the quantity of YB-1 protein and the translocation is closely related to colon cancer cell prolifera-tion differentiation, and could become the new target to colon cancer treatment.