清热解毒方与益生菌联合治疗大鼠脓毒症

Qing Re Jie Du(清热解毒)Prescription Combined Probiotics in Suppressing Main Pathogens to Treat Sepsis

目的:观察清热解毒方和益生菌联合治疗脓毒症模型大鼠的作用。方法:采用盲肠结扎穿孔法制作大鼠脓毒症模型,健康Wistar大鼠140只随机分为假手术组、脓毒症模型组、抗生素组、益生菌组、清热解毒方中药组及清热解毒方和益生菌联合治疗组,对大鼠腹水和血标本进行细菌培养鉴定及实时定量PCR检测。结果:与假手术组比较,脓毒症模型组大鼠腹水和血液中细菌检出率明显升高(P<0.05),其腹水中大肠杆菌检出率100%、金黄色葡萄球菌检出率63.6%,血液中大肠杆菌检出率94.7%、金黄色葡萄球菌检出率21.1%。与模型组比较,单纯中药组和单纯益生菌组治疗后细菌检出率和数量均有所降低(P<0.05)。与单纯用药组比较,联合治疗组上述细菌检出率和数量进一步降低(均P<0.05)。结论:脓毒症模型大鼠主要致病菌为大肠杆菌和金黄色葡萄球菌,清热解毒方和益生菌联合使用可协同抑制其主要致病菌。

Objective To detect the main pathogens in septic model of rats and find the synergism between Qing Re Jie Du（清热解毒）Prescription and Probiotics. Methods The septic model was induced by cecal li-gation and puncture. One hundred and forty healthy Wistar rats were randomly divided into six groups： control group, septic model group, antibiotic treated group, Probiotics treated group, Qing Re Jie Du （清热解毒）Pre-scription treated group and Qing Re Jie Du （清热解毒）Prescription-Probiotics group. Bacterial populations in ascites and blood were determined by bacterial culture and real time PCR. Results The bacterial numbers in model group were significantly increased compared with control data. Detection rates of Escherichia coli and Staphylococcus aureus from ascites were 100% and 63.6% respectively, meanwhile the rates of Escherichia coli and Staphylococcus aureus from blood were 94.7% and 21.1%. The detection rates and bacterial numbers in Qing Re Jie Du （清热解毒）Prescription treated group and Probiotics treated group were decreased compared with those in septic model group. Rates and numbers in Qing Re Jie Du （清热解毒）Prescription-Probiotics group were lower than those in Qing Re Jie Du（清热解毒） Prescription treated group or Probiotics treated group. Conclusion Escherichia coli and Staphylococcus aureus may be primary pathogens of septic rats in our study. Qing Re Jie Du（清热解毒）Prescription-Probiotics treated group synergizes in the treatment of sepsis.