期刊文献+

丙酮酸乙酯对脓毒症大鼠肠组织保护和HMGB1表达的影响

Study of the effect of Ethyl pyruvate on high mobility group Box-1 in septic rats
下载PDF
导出
摘要 目的:通过观察丙酮酸乙酯对脓毒症大鼠肠组织HMGB1表达的影响,进一步揭示丙酮酸乙酯治疗脓毒症的分子作用机制。方法:将96只SD大鼠随机分为假手术组、脓毒症组、低剂量丙酮酸乙酯治疗组(LET组)、高剂量丙酮酸乙酯治疗组(ET组),以盲肠结扎穿刺法复制大鼠脓毒症模型。LET组(20 mg/kg)及ET组(40 mg/kg)即刻腹腔内注射EP注射液4 mL,每6 h重复注射一次,直至实验结束,假手术组及脓毒症组在相同时间用相同剂量的生理盐水腹腔内注射。各组大鼠分别于术后2 h、8 h、24 h、48 h 4个时间点随机处死6只大鼠,经腹主动脉取血,用ELISA方法检测血浆TNF-α、IL-6、HMGB1水平。术后24 h,取大鼠末端回肠,用RT-PCR法检测回肠组织HMGB1mRNA表达水平,用免疫组织化学两步方法观察肠组织HMGB1蛋白表达,在光镜下观察大鼠肠组织的病理变化。结果:与假手术组相比,脓毒症组血浆HMGB1在术后8 h、24 h、48 h显著增高,脓毒症组回肠组织HMGB1mRNA及蛋白表达在术后24 h显著增高(P<0.05)。与脓毒症组相比,ET组、LET组血浆HMGB1在术后8 h、24 h、48 h显著降低,ET组、LET组回肠组织HMGB1mRNA及蛋白表达在术后24h显著降低(P<0.05)。结论:脓毒症大鼠血浆HMGB1出现时间晚,作用时间长,提示HMGB1是脓毒症的重要晚期炎症介质。丙酮酸乙酯可抑制脓毒症大鼠血浆及肠组织HMGB1的表达,提示丙酮酸乙酯对脓毒症的治疗机制可能与其直接或间接抑制HMGB1的表达有关。 Objective: To provide the further evidence for the mechanism of the Dachengqi Ethyl Pyruvate's treatment of sepsis through the observation of the effect of Ethyl Pyruvate on the HMGB1 in septic rats. Methods: we divided 96 SD rats into four group randomly: sham-operation group, septic group, low-dose Ethyl Pyruvate (LET) group and Ethyl Pyruvate intervention (ET) group. The septic model were made by CLP(cecum ligated perforation). The LET group and ET group were injected separately by low dose(20 mg/kg) and normal dose(40 mg/kg) Ethyl Pyruvate injection after CLP and once per 6 hours till the end of the experiment, while the sham-operation group and CLP group were injected by the equivalent normal saline at the same time. Six rats were killed randomly in each group at the point of 2h, 8h, 24h, 48h after CLP. Serum samples were took after centrifugation and the level of TNF-α, IL-6, HMGB1 were tested by ELISA. The intestine tissue were took from the rats killed 24h after the CLP for the test of HMGBlmRNA level of ileum by RT-PCR, the test of HMGB protein level by immunohistochemistry and the observation of rat's pathological changes by optical microscopy. Results: Comparing to sham operation group, the serum and level of HMGB in septic group increased significantly at the point of 8, 24, 48 h after CLP (P〈0.05), and the HMGBImRNA and HMGB1 protein level in septic group increased significantly at the point of 24 h after CLP. Comparing to septic group, the serum and ileal level of HMGB of ET group and LET group significantly 8, 24, 48 h after CLP(P〈0.05), and the HMGBtmRNA and HMGB1 protein level in ET and LET group decreased significantly at the point of 24 h after CLP. Conclusion: The serum level of HMGB1 increased significantly in the septic rats, and its appearance and peak time was later than TNF- α and IL-6's, which prove its character of late inflammatory cytokine in sepsis. As a result of the intervention of Ethyl Pyruvate injection, the serum and ileum HMGB1 level decreased significantly in the septic rats, which probably contribute to the mechanism of treatment of Ethyl Pyruvate injection on sepsis.
出处 《中国现代普通外科进展》 CAS 2014年第3期179-183,共5页 Chinese Journal of Current Advances in General Surgery
关键词 丙酮酸乙酯 脓毒症 高迁移率族蛋白B1 大鼠 High MOBILITY group box-1 Ethyl Pyruvate Sepsis High mobility group box-1 Rat
  • 相关文献

参考文献5

二级参考文献70

共引文献31

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部