CRK1基因缺失对白念珠菌形态、黏附、生物被膜的影响

The effect of CRK1 gene deletion on morphogenesis,adhesion and biofilm formation of Candida albicans

目的研究CRK1基因缺失对白念珠菌形态、黏附、生物被膜的影响。方法显微镜下观察,计算菌丝形成率,比较CRK1基因缺失菌(Δcrk1菌)及标准菌SC5314形成菌丝的能力;建立肠黏膜模型,计算黏附率,评价CRK 1基因缺失对白念珠菌黏附的影响;MTT法及结晶紫法(CV)评价CRK 1基因缺失对白念珠菌生物被膜形成的影响。结果与SC5314相比,Δcrk 1菌分别在10%胎牛血清和RPMI-1640培养条件下形成菌丝能力均较弱,两者之间有统计学差异;Δcrk 1菌在60、90、120 min时对肠黏膜的黏附数明显少于标准菌SC5314,两者之间有统计学差异;通过MTT法、结晶紫法两种方法证实了,在经48 h培养后,Δcrk 1菌与其标准菌SC5314相比,形成生物膜的能力弱,两者之间差异有统计学差异。结论 CRK 1基因缺失影响白念珠菌菌丝二态性的转化,进而影响黏附力和生物被膜的形成。

Objective To observe if the CRK 1 gene deletion influence its virulence of C.albicans.Methods Cells were observed microscopically for formation of hyphae.The numbers of adherent C.albicans were counted by colony counting method after interaction at different time.Biofilm was quantified by MTT assay and CV assay.Results Compared with SC5314,the Δcrk 1 had a weak ability to form hyphae at the condition of 10% Serum and RPMI-1640 medium.The Δcrk 1 had a significant reduction in adhension with human intestinal epithelial cells in vitro at 30,60,90,120 minutes.It was weak for the Δcrk 1 to form biofilm quantified by CV-assay and MTT-assay.Conclusions CRK 1 gene deletion can weaken its morphogenesis,adhesion and biofilm formation.