G-CSF在恶性淋巴瘤患者体内诱导ENA-78和IL-8的作用研究(英文)

Effect of G-CSF on Induction of ENA-78 and IL-8 in the Patients with Malignant Lymphoma

粒细胞集落刺激因子(G-CSF)可以刺激化疗诱导的中性粒细胞减少症患者恢复中性粒细胞计数,并在体外刺激其产生上皮来源的嗜中性粒细胞趋化蛋白-78(ENA-78)和白细胞介素8(IL-8)等趋化因子。本研究旨在探讨G-CSF在体内是否也有类似作用。研究对象为10例接受化疗及G-CSF治疗的淋巴瘤患者,分别在以下时间段采集外周血:化疗前,标记为Time Point(TP1);接受G-CSF治疗前的中性粒细胞减少阶段,标记为TP2;接受G-CSF治疗后的中性粒细胞恢复阶段,标记为TP3。采用实时定量PCR检测中性粒细胞内ENA-78和IL-8的mRNA含量,流式细胞术检测其吞噬功能及产生的活性氧(ROS)。结果表明,在TP2阶段,ENA-78 mRNA表达升高者5例,IL-8 mRNA表达升高者8例;在TP3阶段,ENA-78 mRNA表达升高者3例,降低者6例,IL-8 mRNA表达降低者7例。结论:中性粒细胞内ENA-78和IL-8的表达升高在化疗后中性粒细胞减少的患者体内较常见,而G-CSF干预对其升高无明显作用。

Granulocyte colony stimulating factor （G-CSF） restores neurtrophil count in patients with chemotherapy- induced neutropenia. G-CSF can also induce production of epithelial neutrophil activating protein-78 （ENA-78） and in- terleukin-8 （IL-8）, chemotactic factors from neutrophils in vitro. This study was purposed to investigate whether this effect is also observed in vivo. 10 lymphoma patients were selected who received chemotherapy and G-CSF （ nartogras- tim） administration. Blood was obtained before chemotherapy[ Time Point 1 （ TP1 ） ], at neutropenic phase before G- CSF administration （TP2）, and at neutrophil recovery phase after G-CSF （TP3）. ENA-78 and IL-8 mRNA in neutro- phils were quantified by real-time PCR. Phagocytosis and reactive oxygen species （ROS） generation were examined by flow cytometry. The results showed that ENA-78 and IL-8 mRNA expression at TP2 increased in 5 and 8 patients, re- spectively. The ENA-78 mRNA expression at TP3 was increased in 3 and decreased in 6 patients, and IL-8 mRNA ex- pression at TP3 decreased in 7 patients. G-CSF did not affect phagocytosis and normalized ROS generation in all of the patient. It is concluded that increase of ENA-78 and IL-8 expression in neutrophils is common in chemotherapy-induced neutropenic patients. G-CSF administration does not significantly increase ENA-78 and IL-8 expression.