右旋蛋氨酸对小鼠造血系统辐射损伤的作用

Effects of D-methionine on the Hematopoietic System Injury in Irradiated Mice

本研究旨在探讨右旋蛋氨酸(D-methionine,D-met)对造血系统辐射损伤的影响。将C57BL/6小鼠分为对照组、照射组、D-met 300 mg/kg组+照射组和D-met 1000 mg/kg+照射组。对照组小鼠接受假照射,其余3组小鼠接受7.5 Gy,1、4、8 Gy和1 Gyγ线的全身照射,分别用于小鼠存活率、骨髓细胞存活率、外周血白细胞数及其分类数以及骨髓细胞集落形成能力的检测。D-met于照射前30 min腹腔注射。结果表明,300和1000 mg/kg D-met均未显著提高7.5 Gy照射小鼠的存活率;10-2、10-3和10-4mol/L D-met能明显提高1、4、8 Gy照射小鼠骨髓细胞的存活率;300和1000 mg/kg D-met虽能使受1 Gy全身照射小鼠的外周血白细胞数有所增加,但与照射小鼠相比却无统计学差异,而对骨髓细胞集落形成能力具有明显的促进作用。结论:D-met可有效缓解辐射对骨髓细胞的杀伤作用,提高受照小鼠骨髓细胞的存活率,促进照射小鼠造血功能的恢复。

The aim of this study was to investigate the effects of D-methionine（D-met） on the hematopoietic system injury in irradiated mice. C57BL/6 mice were divided into control group, irradiated group, 300 mg/kg D-met plus irra- diation group and 1000 mg/kg D-met plus irradiation group. The control mice received sham irradiation, and the mice in remainder groups were exposed to 7.5 Gy; 1,4,8 Gy and 1 Gy of 137Cs γ-ray respectively, were used to detect the sur- vival rate, survival rate of bone marrow cells, WBC and its differential counts as well the colony formation ability in ir- radiated mice, respectively. The D-met was intraperitoneally injected to mice at 30 min before irradiation. The results showed that 300 and 1000 mg/kd D-met did not obviously enhance the survival rate of mice exposed to 7.5 Gy; the 10-2,10-3,10-4 mol/L D-met significantly increased the survival rate of bone marrow cells in mice exposed to 1,4,8 Gy; 300 and 1000 mg/kg D-met even so increased the WBC count of peripheral blood in mice exposed to 1 Gy, but there was no statistical difference as compared with irradiated alone mice, moreover 300 and 1000 mg/kg D-met could obviously promote the colony formation ability of bone marrow cells in irradiated mice, the CFU-GM count was higher than that in 1 Gy irradiated mice （P 〈 0.05 ）. It is concluded that the D-met can effectively mitigate the marrow cell in- jury resulted from irradiation, enhance the survival rate of bone marrow cells in irradiated mice, promote the recovery of hematopoietic function from radiation injury in mice.