GRP78在胃癌生长中的作用

Central role of GRP78 in growth of gastric carcinoma cells

目的:探讨内质网分子伴侣葡萄糖调节蛋白78(GRP78)参与胃癌细胞生长的作用。方法:回顾性分析34例胃癌及34例癌旁组织,利用组织微阵列技术,构建组织阵列,采用免疫组织化学技术SP法检测该阵列中GRP78蛋白在胃癌及其癌旁组织中表达情况。利用Western blotting检测人体胃癌组织、癌旁组织(肿瘤旁1cm处)和正常组织(远离肿瘤≥10 cm处)中GRP78蛋白的表达情况。利用Western blotting检测人胃癌细胞SGC7901和过表达GRP78的SGC7901-H78细胞(稳定转染GRP78)中GRP78和生长相关蛋白cyclin D1的表达情况。结果:(1)免疫组织化学检测发现:人体胃癌组织中GRP78表达水平明显高于癌旁组织和正常组织,且与性别、分化程度相关(P<0.05);(2)Western blotting检测发现:胃癌组织中GRP78蛋白的表达明显高于癌旁组织和正常组织;(3)Western blotting检测发现:相对于SGC7901细胞,SGC7901-H78细胞中GRP78蛋白的表达明显升高,同时检测生长相关蛋白cyclin D1的表达发现,随着GRP78表达的上调,cyclin D1蛋白表达增加。结论:GRP78蛋白的高表达可能参与了胃癌细胞的生长。

AIM ： To explore the effect of glucose-regulated protein 78 （GRP78） on the gastric carcinogenesis. METHODS： GPR78 expression patterns were examined in 34 specimens from gastric carcinoma patients using the immu- nohistochemistry （IHC） assay, and in 10 specimens using Western blotting analysis. In addition, the expression of GPR78 and cyclin D1 was detected in human gastric cancer cell lines SGC7901 and SGC7901-H78 （overexpressing GRP78） by Western blotting. RESULTS ： By IHC assay, GRP78 was found to be highly expressed in the cytoplasm of gastric carcino- mas as compared with the adjacent non-malignant tissues and corresponding normal tissues. GRP78 expression was positive- ly correlated with gender and histological differentiation （ P 〈 0. 05）, but not with age, tumor stage and lymph node metas- tasis （ P 〉 0. 05）. Furthermore, we found that with the increased expression of GRP78 in SGC7901-H78 cells, the expres- sion of cyclin D1 was also elevated. CONCLUSION： GRP78 might be a key player to be involved in the growth of gastric