正交试验优选血三七总黄酮的提取工艺研究

Extraction optimization of total flavonoids from Polygonum amplexicaule var. sinense by orthogonal test

目的优选血三七Polygonum amplexicaule var.sinense中总黄酮的提取工艺。方法以乙醇体积分数、提取时间、乙醇倍数、提取次数为因素,以总黄酮提取率和浸膏得率为指标,采用正交试验优选提取工艺。结果最佳条件为用10倍70%乙醇,提取3次,每次1.5 h。结论优选的工艺稳定、合理,可用于血三七中总黄酮的提取。

Objective To optimizetheextractiontechnology of total flavonoids from Polygonum amplexicaule var.sinense.Methods The extraction technology of total flavonoids was optimized by orthogonal test using ethanol concentration, ethanol ratio, extracting time, and extracting times as factors with the extraction of total flavone and extract yield as indexes. Results The optimal extraction technology was as follows： 10-folds of 70% ethanol, extracting for three times, and 1.5 h each time. Conclusions The optimal technology is stable and feasible for the extraction of total flavonoids from P. amplexicaule var. sinense.