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HPLC-DAD法测定苦碟子中木犀草素7-O-β-D-葡萄糖苷、木犀草素7-O-β-D-葡萄糖醛酸苷和芹菜素7-O-β-D-葡萄糖醛酸苷 被引量:8

Determination of luteolin-7-O-β-D-glucopyranoside, luteolin-7-O-β-D-glucuronopyranoside, and apigenin-7-O-β-D-glucuronopyranoside in Ixeris sonchifolia by HPLC-DAD
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摘要 目的利用HPLC-DAD法建立苦碟子中木犀草素7-O-β-D-葡萄糖苷(LGCOP)、木犀草素7-O-β-D-葡萄糖醛酸苷(LGCRP)和芹菜素7-O-β-D-葡萄糖醛酸苷(AGCRP)的测定方法。方法 Diamonsil C18色谱柱(200 mm×4.6 mm,5μm);流动相为(0.05%甲酸–水)–(0.05%甲酸–乙腈);二元梯度洗脱:检测波长340 nm;体积流量1.0 mL/min;柱温35℃;进样量10μL。结果在选定色谱条件下线性关系良好(r≥0.999 9)。平均回收率分别为100.5%、100.1%、100.8%,RSD值分别为0.4%、0.3%、0.7%。结论该分析方法能简便、快速地测定苦碟子中黄酮类成分,可为评价不同产地、药用部位及采收期的药材提供科学依据。 Objective To establish an analysis method for simultaneous determination of luteolin7-O-β-D-glucopyranoside (LGCOP), luteolin7-O-β-D-glucuronopyranoside (LGCRP) and apigenin7-O-β-D-glucuronopyranoside (AGCRP) in lxeris sonchifolia by HPLC-DAD. Methods HPLC was carried out on a Diamonsil Ci8 ( 200 mm ×4.6mm, 5 μm) column with (0.05% formic acid- water) - (0.05% formic acid - acetonitrile) as mobile phase for two elements of gradient elution. The detection wavelength was set at 340 nm. The injection volume was 10 μL at the flow rate of 1.0 mL/min. The temperature of column was set at 35 ℃. Results There were good linear relationship ofLGCOP, LGCRP, and AGCRP (r≥0.999 9). The average recoveries were 100.5%, 100.1%, and 100.8% with RSD values of 0.4%, 0.3%, and 0.7%, respectively. Conclusion The method is convenient, specific, and can be used for the quality control of flavonoids in L sonchifolia from different areas, various medicinal parts, and different harvest.
出处 《现代药物与临床》 CAS 2014年第4期377-380,共4页 Drugs & Clinic
基金 国家科技重大专项(2011ZX09401-305-42) 国家自然科学基金青年基金项目(81102732)
关键词 苦碟子 黄酮类成分 木犀草素7-O-β-D-葡萄糖苷 木犀草素7-O-β-D-葡萄糖醛酸苷 芹菜素7-O-β-D-葡萄糖醛酸苷 HPLC—DAD Ixeris sonchifolia (Bge.) Hance flavonoids luteolin-7-O-β-D-glucopyranoside luteolin-7-O-β-D-glucuronopyranoside apigenin-7-O-β-D-glu-curonopyranoside HPLC-DAD
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