HPLC法测定津蓟中风Ⅰ号颗粒中梓醇和五味子醇甲

Determination of catalpol and schisandrin in Jinji Zhongfeng No. 1 Granules by HPLC

目的建立津蓟中风I号颗粒中梓醇和五味子醇甲的HPLC测定方法。方法采用HPLC法分别对梓醇、五味子醇甲进行测定。梓醇的流动相为乙腈-0．1％磷酸（1：99），体积流量为1．0mL／min，检测波长为210nm。五味子醇甲的流动相为甲醇一水（75：25），体积流量为0．8mL／min，检测波长为250nm。结果梓醇在4．25~68．00μg／mL与峰面积线性关系良好，平均回收率为100．22％，RSD值为1．05％（n=9）。五味子醇甲在4．0～32．0rtg／mL与峰面积线性关系良好，平均回收率为100．49％，RSD值为1．25％（n=9）。结论本法简便、准确性高、灵敏度高、专属性和重现性好，可作为津蓟中风I号颗粒中梓醇和五味子醇甲的测定方法。

Objective To establish a method for the determination of schisandrin and catalpol in Jinji Zhongfeng No. 1 Granules. Methods Catalpol and schisandrin were determined by HPLC method, respectively. Catalpol was determined with acetonitrile - 0.1% phosphoric acid （1 ： 99） as the mobile phase, the flow rate was 1.0 mL/min, and the detection wavelength was 210 nm. Schisandrin was determined by methanol - water （75： 25） as the mobile phase, the flow rate was 0.8 mL/min, and the detection wavelength was 250 nm. Results The liner ranges of catalpol and schisandrin were 4.25 -- 68.00 pg/mL （r=0.999 9）, 4.00 -- 32.00 μg/mL （r=0.999 9）. The average recoveries were 100.22%,and 100.49%, with RSD values of 1.05%,and 1.25% （n=9）, respectively. Conclusion The method is specific, accurate and reproducible, and can be used for the quality control of catalpol and schisandrin for Jinji Zhongfeng No. 1 Granules.