甘肃省不同地区大麦条纹病病菌致病力测定及RAPD分析

Pathogenicity determination and RAPD analysis of Drechslera graminea solations in different area in Gansu province

通过对从甘肃的张掖、武威、天祝、秦王川、甘南等地的大麦和青稞叶片及种子上共分离得到的11个条纹病病菌菌株进行致病力测定。结果显示,不同菌株在致病力等方面存在明显差异。其中菌株TZ1和LQ1致病力最强,离体叶片接种和拌种接种在大麦叶片上发病率均达到80%以上,显著高于其他菌株。将这11个菌株基因组DNA经RAPD分析表明,不同菌株间的扩增图谱差别明显,表现出各菌株间明显的遗传多样性。经聚类分析显示,大麦条纹病病菌不同菌株的RAPD类群与致病力和不同寄主有一定的相关性。

Eleven isolations of Drechslera graminea were obtained from diseased leaves and seeds collected from Zhangye, Wuwei, Tianzhu , Qinwangchuan and other regions in Gansu province. There were significant differences in pathogenicity among different isolations. All isolations TZ1 and LQ1 strains had most virulence of which diseased leaf rate were above 80% after being inoculated onto separating barley leaves in room. Pathogenicity of TZ1 and LQ1 strains were significantly greater than those of the others. There was obvious difference in RAPD amplification patterns among 11 isolations demon- strating their genetic polymorphism, and there were relationship between RAPD group of D. graminea and the pathogenici- ty in different hosts.