激光拉曼光谱法无损鉴别三七及其伪品

Nondestructive Identification of Panax Notoginseng and Its Analogues via Laser Raman Spectroscopy

应用激光拉曼光谱技术准确无损鉴别三七及其伪品菊三七、藤三七和姜黄。采集了三七及其伪品的拉曼光谱图,并结合二阶导数拉曼图谱对其进行定性鉴别。四种药材的图谱中均出现了1330、1040、943、310 cm-1糖类碳水化合物的拉曼特征峰。三七的谱图中出现了1080 cm-1和1120 cm-1皂苷类化合物的拉曼振动峰;菊三七的谱图中出现了820、899、1144、2935 cm-1b-谷甾醇、吡咯里西啶类生物碱及长链脂肪烃的拉曼特征峰;藤三七的谱图中出现了1462 cm-1和1695 cm-1的腺苷类和尿嘧啶类化合物的拉曼振动峰;姜黄的谱图中出现了962、1187、1433、1596、1630 cm-1等姜黄素类化合物的拉曼振动峰。以上专属特征峰的差异可将三七及其伪品进行很好的区分,并据谱图信息可建立四味中药的拉曼指纹图谱。二阶导数拉曼光谱图可以对三七及其伪品的鉴别结果进行进一步补充验证说明。此方法可准确直接、快速鉴别三七及其伪品。

Panax Notoginseng and its analogues such as Gynura Segetum, Boussingaultia Gracilis and Curcuma Longa are indentified via Raman spectra technique. The characteristic Raman spectra of Panax Notoginseng and its analogues are acquired, then they are indentified by laser Raman spectroscopy and second derivative Raman spectroscopy. The structural information indicates that Panax Notoginseng and its analogues contain some carbohydrate substrates, due to some characteristic vibration peaks of carbohydrate moiety, because 1330, 1040, 943 and 310 cm^-1： characteristic absorption peaks can be observed. The characteristic vibration peaks at 1080 cm^-1 and 1120 cm^-1indicate the existence of saponin compounds. Chrysanthemum Notoginseng shows characteristic vibration peaks at 820, 899, 1144 and 2935 cm^-1 because of the presence of β-sitosteroland, pyrrolizidine alkaloids compounds and long chain aliphatic compounds. The characteristic vibration peaks at 1462 cm^-1 and 1695 cm^-1 appear in Rattan Notoginseng spectroscopy, because of the existenence of adenosine compounds and uracil compounds. The characteristics vibration peaks of Rhizoma Curcumae Longae, such as 962, 1187, 1433, 1596 and 1630cm-＇can also be observed due to some curcumin compounds. The difference of absorption peaks between Panax Notoginseng and its analogues can be used to distinguish them. Finger spectroscopy of Panax Notoginseng and its analogues can be further established, the identification results can be further verified via second derivative Raman spectroscopy. The above method can be easily used to provide a more accurate, direct and fast identification of Panax Notoginseng and its analogues.