调脂积冲剂对高脂诱导肥胖大鼠脂联素、抵抗素影响的研究

Study about Adiponectin and Resistin Level of Obese Rats Caused by High-Fat under Tiaozhiji Intervention

目的:通过高脂诱导营养性肥胖大鼠模型,观察调脂积冲剂对肥胖大鼠体重、Lee’s指数、血清胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白(HDL)、低密度脂蛋白(LDL)、血糖(FBG)、真胰岛素(FTI)、胰岛素抵抗指数(HOMA-IR)及游离脂肪酸(FFA)、脂联素、抵抗素的影响。方法:将SD雄性大鼠65只,分为正常组10只,其余55只采用高脂饮食建立肥胖模型。7周末时测体重,大鼠体重大于正常组体重20%为造模成功。将造模成功的30只大鼠随机分为模型组、调脂积组、荷丹片组。灌胃给药5周后,测量各组大鼠体质量、身长,计算Lee’s指数。检测各组大鼠血清TC、TG、HDL、LDL、FBG、FFA。采用ELISA法测定血清的真胰岛素、脂联素、抵抗素含量。采用稳态模式评估法(HOMA)来评价胰岛B细胞功能,即胰岛素抵抗指数(HOMA-IR)=(FBG)*FTI/22.5。结果:和正常组比较,模型组大鼠体重、Lee’s指数、TC、TG、LDL、FTI、HOMA-IR、FFA、抵抗素明显升高(P<0.01);FBG有差异性升高(P<0.05);HDL有差异性降低(P<0.05);脂联素明显降低(P<0.01)。和模型组比较,调脂积组大鼠体重明显下降(P<0.01);Lee’s指数、TG有差异性下降(P<0.05);TC、LDL明显下降(P<0.01);FBG略有下降、HDL略有升高,但无统计学意义(P>0.05);HOMA-IR、FTI、FFA明显下降(P<0.01);血清脂联素有差异性升高(P<0.05);血清抵抗素有差异性下降(P<0.05)。调脂积组和荷丹片组比较,在上述指标方面差异无统计学意义(P>0.05)。结论:高脂饲料诱导的肥胖大鼠体内存在胰岛素抵抗,血清FFA、抵抗素水平升高,脂联素水平降低;调脂积冲剂可以减轻肥胖大鼠的体重,调节血脂紊乱,改善其胰岛素敏感性,这种作用可能和降低血清FFA、抵抗素水平及升高脂联素水平有关。

Objective： The obese rat model was induced by the high fat diet. To observe the influence of Tiaozhiji on weight of obese rat model, Lee＇s index, serum cholesterol （ TC）, triglyceride （ TG）, high density lipoprotein （ HDE）, low density lipoprotein （LDL） ,blood glucose （FBG） ,true insulin （ FTI）, insulin resistance index （ HOMA - IR）, and free fatty acid （ FFA）, adiponectin, resistin changes. To explore the mechanism of action of Tiaozhiji affecting body weight and insulin sensitivity in rat model. Methods： 65 male SD rats were divided into two groups, 10 of the normal group,the remai- ning 55 were made the obesity model by high fat diet. The body weight of rats was measured after 7 weeks. The weight 20% greater than the normal indicated modeling success. 30 successful modeling rats were randomly divided into model group,Tiaozhiji group and Hedan group. Gavage for 5 weeks,the rats body weight,length,calculation of Lee~ index were measured. Each group was detected the serum TC, TG, HDL - C and LDL - C, FBG and FFA. The enzyme - linked immu- nosorbent assay was used for determination of serum true insulin, adiponectin, resistin content. The homeostasis model as- sessment （HOMA） was used to assess the islet B cell function,insulin resistance index （HOMA -IR） = （FBG） × FTI/ 22. 5. Results ： By comparison, the body weight of rats of model group was significantly increased than that in the normal group. Lee＇s index, TC, TG and LDL, FTI and HOMA -IR, FFA and resistin were significantly increased （P 〈 0. 01 ）. FBG were increased in different degrees （ P 〈 0.05 ）. HDL was decreased （ P 〈 0. 05 ）. Comparison of model control group, the body weight of rats in the Tiaozhiji group was decreased significantly （P 〈 0.01 ）. Lee＇s index and TG were de- creased significantly （ P 〈 0. 05）. TC and LDL were decreased significantly （P 〈 0. 01 ）. FBG was slightly decreased and HDL increased slightly but there was no statistical significance （ P 〉 0. 05 ）. HOMA - IR, FTI and FFA were decreased significantly （ P 〈 0.01 ）. Serum adiponectin was increased significantly （ P 〈 0.05 ）. The serum resistance was signifi- cantly decreased （P 〈 0.05）. Tiaozhiji group and Hedan group had no significant differences in these indicators （ P 〉 0. 05 ）. Conclusion： The high fat diet - induced obese rats had insulin resistance, and the increased serum FFA and resistin levels and lower adiponectin levels. Tiaozhiji can reduce obese rats body weight, regulate blood lipid disorders, and im-prove their insulin sensitivity. This effect may be related with reducing serum FFA, resistin and increasing adiponectin.