中药育泡饮对大鼠卵巢颗粒细胞P38MAPK表达的影响

Effect of Yupao Decoction on Protein Expression of P38MAPK of Rat's Ovarian Granulosa Cells

目的:观察中药育泡饮对体外培养大鼠卵巢颗粒细胞P38MAPK表达的影响。方法:颗粒细胞预培养48 h后,随机分组,加入促卵泡激素FSH(50 ng/mL)和不同剂量的育泡饮,并设立空白对照组,继续培养48 h,Western-Blot法检测各组颗粒细胞P38MAPK的表达。结果:与空白组相比较,育泡饮各剂量组及FSH组均能促进P38MAPK表达(P﹤0.05)。与FSH组比较,除了低剂量组育泡饮P38MAPK表达明显降低(P﹤0.05),中、高剂量组P38MAPK表达与之无显著性差异(P﹥0.05)。结论:育泡饮通过激活颗粒细胞P38MAPK通路,可能参与了对卵泡发育、成熟的调控。

Objective ： To observe Yupao Decoction on the protein expression of P38MAPK of rat＇ s ovarian Granulosa cells （GC）. Methods ： GCs were isolated from rat＇ s ovaries and cultured for 48 hours and were divided into five groups ： three dosage groups of Yupao Decoction,blank control group and FSH （50 ng/mL） control group at random. After cul- tured for 48 h,the protein expression of P38MAPK of each group GC was detected by Western- Blot method. Result： Three dosage groups of Yupao Decoction and FSH control group all could promote P38MAPK expression. The difference was remarkable comparing with the blank control group （ P 〈 0.05 ）. Compared with FHS control group, it only de- creased in low- dosage group. The P38MAPK expression in medium and high- dosage groups had no difference （ P 〉 0.05 ）. Conclusion ： Yupao Decoction may be involved in the regulation of follicular growth and maturation by activating P38MAPK pathway.