高效液相色谱法测定复方黑参颗粒中射干苷的含量

Determination of Content of Belamcandin in Compound Heishen Granules by HPLC

目的：建立HPLC法测定复方黑参颗粒中射干苷的含量。方法：色谱柱：DiamonsiffMC18色谱柱（250mm×4．6mm，5μm）；流动相：乙腈-0．05％磷酸，梯度洗脱；流速：1．0mL／min，检测波长：266nm；柱温：30℃。结果：射干苷在21．12—105．6μg／mL范围内线性关系良好（r=0．9998）。平均加样回收率为98．3％，RSD为1．7％（n=9）。结论：该方法简便快速，适用于复方黑参颗粒中射干苷的含量测定。

Objective： To establish a method for the determination of helamcandin in compound Heishen Granules by HPLC. Methods： The separation was performed on a Diamonsil^TMC18 column （ 250 mm x4.6 mm,5 μm ） with a gradient elution composed of acetonitrile and 0.05% phosphoricacid. The flow rate was 1.0 mL/min. The detection wavelength was 266 nm and the column temperature was 30℃. Result： The calibration curve was linear（ r = 0. 9998） in the range of 21.12 - 105.6 μg/mL for belamcandin. The average recovery of belamcandin in compound Heishen Granules was 98. 3% （ n =9）. Conclusion： The method is quick and convenient for the determination of belamcandin in compound Heishe Granules.