HPLC法同时测定芪黄疏糖胶囊中梓醇和毛蕊花糖苷的含量

Simultaneous Determination of Catalpol and Verbascoside in Qihuang Shutang Capsule by RP-HPLC

目的：建立芪黄疏糖胶囊中梓醇和毛蕊花糖苷含量测定方法，为其他含地黄制剂含量测定提供参考。。方法：色谱柱为AgilentHC—C18（250mm×4．6mm，5μm）；以乙腈-0．1％磷酸溶液为流动相，梯度洗脱；检测波长在210nm和334nm间切换；流速1．0mL/min；柱温28℃。结果：梓醇进样量在0．385～11．55μg、毛蕊花糖苷进样量在0．115—3．45μg范围内呈良好线性关系。结论：该方法准确度和重复性好，适用于芪黄疏糖胶囊中生地黄的含量测定。

Objective： To establish a method for simultaneously quantification of the catalpol and verbascoside in Qi- huang Shutang capsule and to provide the reference for other content determination of containing rehmannia prepation. Methods： RP -HPLC was performed on Agilent HC -C18 column （250 mm x 4.6 mm,5 μm） with acetonitrile - 0.1% phosphoric acid aqueous solution as mobile phase, gradient elution, detection wavelength was switched at 210 nm and 334 rim, flow rate was 1.0 mL/min, column temperature was 28 ℃. Results： A good linear relationship was obtained from 0.385 -11.55 μg and 0. 115 -3.45 μg for catalpol and verbascoside, respectively. Conclusion： The results show that this method is accurate and reproducible for content determination of rehmanniae in Qihuang Shutang capsule.