在高糖条件下锰超氧化物歧化酶刺激MSC提高血管内皮细胞活性的研究

The research of manganese superoxide dismutase stimulating MSC to improve the activity of vascular endothhheial cells under the conditions of high glucose

目的观察高糖环境下,锰超氧化物歧化酶(MnSOD)刺激间充质干细胞(MSC)上清液对人脐静脉血管内皮细胞(HUVEC)增殖和趋化能力的影响。方法高糖MSC培养基条件下,100μg/L MnSOD刺激的MSC为实验组,未刺激的MSC为对照组,添加Akt阻断剂5μmol/L Tricirlbine为Akt阻断剂组,利用各组条件培养基在高糖1640培养基条件下,培养人脐静脉内皮细胞(HUVEC),使用MTT法观察各条件培养基对HUVEC增殖的影响,并利用细胞划痕实验和Transwell细胞迁移实验对HUVEC的趋化情况进行评估。结果与对照组相比,MSC实验组条件培养基促进HUVEC增殖;但是Akt阻断剂组HUVEC增殖能力降低;MSC实验组条件培养基作用的HUVEC趋化活动与对照组相比明显提高,但MSC实验组HUVEC趋化活动在Akt阻断剂组明显被抑制;MSC实验组条件培养基中VEGF含量比对照组明显提高。结论高糖条件下MnSOD可以通过刺激间充质干细胞旁分泌VEGF等细胞因子对血管内皮细胞增殖、趋化等能力产生影响。

Objective To observe the influence of proliferation and chemotactic capability of HUVEC of MSC super- natant fluid stimulating by MnSOD. Methods Under the condition of high sugar MSC medium, taking the MSC stimu- lated by lO0p, g,/L MnSOD as the experiment group, the unstimulated MSC as the control group. HUVEC, using cell scratch experiments and transwell cell migration experiments to make an evaluation for chemokines case of HUVEC. Results Compared with the control group, the conditions medium of MSC experiment group to promote the proliferation of HUVEC; but the chemotactic activity of HUVEC of MSC experiment group was inhibited significantly in the group of Akt inhibitor; the contents of VEGF in the conditioned medium of MSC experimental group was significantly higher than the control group. Conclusion Under the condition of high sugar, by stimulating MSC paracrine VEGF and other cell factors, MnSOD can have some influence on proliferation and chcmotactic ability of cytokines on vascular en- dothelial cells.