甘蔗胚状体的诱导与植株再生

EMBRYOID INDUCEMENT AND PLANT REGENERATION IN SUGARCANE

甘蔗组织培养中,2,4-D是外植体脱分化产生胚性愈伤组织并进一步诱导出胚状体的关健因素。2,4-D浓度为10μm时,从外植体上产生的愈伤组织可直接诱导出胚状体;2,4-D浓度为5μm时,可诱导外植体直接产生胚状体。Dropp0.5mg/L(TDZ1.14um)对已继代3、4次的愈伤组织诱导产生胚状体的效果极佳。

In the tissue culture of sugarcane,2,4-D(2,4-dichlorphenoxyacetic acid) was the key factor to induce somatic embryogenesis in sugarcane expla-nts. The somatic embryoid could be initiated from the callus of explant when the concentration of 2,4-D was 10um. The somatic embryoid could be immediately intiated from the leaf explants and transformed into regenerated plantlets when 2,4-D was supplemented to media at 5um. TDZ at 1.14um was very advantagenous to produce somatic embryoid on the callus subeultured 3 and 4 times.