摘要
中国黄连细胞无性系无论进行固体培养或液体悬浮培养,均以6,7-V培养基上的培养物生长速度快,小檗碱含量高。在6,7-V培养基的基础上,以蔗糖(试剂)或食用砂糖作碳源的培养物,其生长速度、干物质积累与小檗碱含量比萄葡糖或麦芽糖作碳源的高。适宜培养温度为22～25℃。
The solid culture and liquid suspension culture of Copiis Chinensis both grew quickly and accumulated more berberine on 6.7-V medium. As a carbon source based on 6.7-V medium, sucrose (reagent) and edible granulated sugar both were better than glucose or maltose, according to culture growth, drymatter prodution and berberine accumulation. The suitable culture temperature was 22-25℃.
出处
《贵州农业科学》
CAS
1991年第2期1-4,共4页
Guizhou Agricultural Sciences
基金
贵州省科技基金资助项目内容之一
关键词
中国黄连
固体培养
液体悬浮培养
Copiis Chinensis
solid culture
liquid suspension culture
berberine
culture conditions
