当归补血汤治疗癌性贫血的实验研究

Research of Angelica Blood Tonic Soup in Treatment of Carcinoma of Anemia

目的:研究不同浓度当归补血汤对荷瘤小鼠补血作用。方法:建立生长良好的H22小鼠的腹水模型,将荷瘤小鼠随机分为2组(模型组、当归补血汤组),另外再选12只健康小鼠为空白对照组(正常组)。给予正常组和模型组小鼠每日灌胃生理盐水,当归补血汤组给予当归补血汤灌胃(剂量为12g·kg·d-1)。分组后连续给药10d,于末次给药24h眼球采血后处死小鼠收集骨髓。结果:实验数据表明,与正常组相比,模型组红细胞数及血红蛋白含量明显减少,提示可能存在癌性贫血现象。当归补血汤组相比模型组其红细胞数、血红蛋白有所上升,提示当归补血汤可能对抗癌性贫血。将各组骨髓细胞体外培养,当归补血汤组能减缓癌性贫血小鼠骨髓红系祖细胞的凋亡速度。结论:当归补血汤对荷瘤小鼠有一定补血作用。

Clinical studies have shown that cancer more than 50% incidence of anemia, anemia can affect the tumor treatment, reduce the patients quality of life, even life threatening. Objective： to study the different concentration of angelica blood tonic soup on a tumor -bur dened mice blood tonic effect. Methods ： H22 mice ascites of good growth model is set up, a tumor - burdened mice were randomly divided into two groups （model group, angelica blood tonic soup）, in addition to choose 12 healthy mice as blank control group （normal group）. Give the normal group and model group mice lavage daily physiological saline, angelica group was given angelica blood blood tonic soup decoction lavage （ dose of 12 g . kg . d^-1 ）. Grouping after dosing 10 days in a row, on time delivery at the end of the 24 h after eyeball blood executed collect in mice bone marrow. Resuhs： the experimental data show that, compared with normal group, model group signifi cantly reduced number of red blood cell and hemoglobin content and suggest possible cancer anemia phenomenon. Angelica blood tonic soup group compared with model group the number of red blood ceils, hemoglobin increased, and suggest possible angelica blood tonic soup for cancer anemia. Groups of bone marrow cells in vitro, angelica blood tonic soup group can slow cancer anemia mice bone marrow apoptosis velocity of red blood cell precursors. Conclusion： angelica blood tonic soup for a tumor -burdened mice has a role in blood.