龟板提取物调控骨髓间充质干细胞中Id1表达的研究

Extracts from Plastrum Testudinis regulates the expression of Id1 in MSCs

目的观察龟板提取物在不同的时间点对骨髓间充质干细胞中分化抑制蛋白1的影响。方法将构建成功的PGL3-Id1启动子用磷酸钙共沉淀法转染大鼠骨髓间充质干细胞,龟板提取物分别作用于转染后的骨髓间充质干细胞36小时、3天、7天,每个时间点分别加入0,1,3,30,100μg/ml的龟板提取物,药物作用一定时间后收集细胞分别应用萤光素酶报告基因系统、RT-PCR检测Id1蛋白的表达。结果 36小时和3天时,小剂量的龟板提取物对分化抑制蛋白1的影响不大,随着时间和剂量的增加,龟板提取物量效依赖性地促进了Id1的表达;7天时,龟板提取物对Id1的促进作用逐渐减弱。结论龟板提取物促进了骨髓间充质干细胞中分化抑制蛋白1的表达,且其促进作用呈现增高-最强-减弱的特点。

Objective To explore Extracts from Plastrum Testudinis （PTE） regulates expression of Idl in different times in MSCs. Methods A SD rat PGL3 - Idl promoter sequence had been constructed. MSCs would be transfected with the calcium phos- phate co - precipitation method. Transfected cells were harvested 36 hours, 3 days and 7 days respectively. Different dosages of PTE 0 ,1,3 ,30 ,100μg/mL were used to active the transfected MSCs in different time point respectively. Then luciferase activity measurement and RT - PCR were used to detect the activity of Idl. Results Low doses of PTE few active the activity of Idl and this promotion is more and more remarkable along with increasing doses or time in 36 hours and 3 days. When it is 7 days, the promotion of PTE effecting MSCs has become less. Conclusion PTE increases the expression of Idl in MSCs. This promotion pres- ents the feature of increase - highest - weakening.