超氧化物歧化酶微胶囊的制备及微囊结构的表征

Preparation and in vitro Characterization of Superoxide Dismutase Microcapsules

[目的]优化超氧化物歧化酶微胶囊的制备工艺,并对该微胶囊的结构进行初步的分析。[方法]采用水中干燥法制备超氧化物歧化酶微胶囊,并对其工艺进行优化。利用扫描电镜技术,分析超氧化物歧化酶微囊的表面形态及大小分布;并采用X射线光电子能谱和示差扫描热量技术,初步研究超氧化物歧化酶微囊的结构。[结果]在最佳工艺条件下,超氧化物歧化酶微囊形状规则,粒径分布均匀。超氧化物歧化酶微胶囊是由乙基纤维素包裹超氧化物歧化酶成囊的,乙基纤维素与超氧化物歧化酶呈囊后,乙基纤维素与超氧化物歧化酶分子间形成很强的分子间相互作用。[结论]超氧化物歧化酶微囊的结构分析,对进一步研究微胶囊的控制释放机制和改进微囊性能具有一定的意义。

[ Objective] The major purpose of this research is to characterize the structure of SOD microcapsules in vitro. [ Method] First, the preparation of SOD microcapsules was carried out using ethylcellulose by the method of drying in water. With the scanning electron microscope, the morphologies of superoxide dismutase microcapsules were investigated. And then, with the Xray photoelectron spectroscopy and different scanning calorimetry technology, the structures of SOD microcapsules was further characterized. [ Result] Our results showed that the SOD mi- crocapsules exhibited regular shapes and uniform particle size distribution. The results showed that strong inter-molecular interactions between ethylcellulose and SOD existed in the microcapsules when the SOD microcapsules were wrapped by ethylcellulose, This was the first report of the structure of SOD microcapsules. [ Conclusion] Thus, this study has great meanings to understand the controlled release mechanism of the micro- capsules and further improve the performances of SOS microcaDsules in the future.