抗肿瘤活性青蛤多肽的提取工艺研究

Anticancer Activity of Peptides Isolated from Hydrolysates of Cylcina sinensis

[目的]探究具有抗肿瘤活性的青蛤多肽的最佳提取工艺路线.[方法]选择胰蛋白酶酶解青蛤,以酶解液对PC-3细胞增殖抑制率（IR）为指标,研究料液比、温度、pH、加酶量和酶解时间对多肽提取率的影响,探讨最佳工艺,并采用超滤及G25洗脱分离纯化提取液.[结果]当温度为45℃、pH为7.0、料液比为1∶3、酶解时间为6h、加酶量为300 U/g时,分子量为3～5 kD青蛤提取液的抗肿瘤活性最好;分离纯化后在280 nm波长处得到3个峰,其中峰1和峰2成分抗肿瘤活性显著.[结论]以细胞增殖抑制率为指标可以确定青蛤多肽的提取及优化工艺路线.

[ Objective ] To explore optimal extraction process for Cylcina sinensis peptides. [ Methods ] Cylcina sinensis was hydrolyzed with trypsin to get peptides; the conditions of hydrolysis were optimized according to its antitumor activity on PC-3 cell; solid to liquid ratio, extrac- tion times, temperature, pH and enzyme concentration were studied ; the technology parameters was determined by the orthogonal test. Cylcina sinensis hydrolysates were isolated by uhra-filtration and purified using G-25 gel filtration. [ Results] The best conditions of enzymatic hydrolysis were temperature of 45 ℃, pH 7.0, enzyme concentration of 300 U/g, solid-hquid ratio of 1：3, and time of 6 h. After gel filtration, three an- ticancer peptide fractions were collected. Peak 1 and peak 2 had the higher anticancer activity. [ Conclusion] Taking inhibition of cell prolifer- ation as indicators can effectively extract clam Cylcina sinensis peptides and optimize process routes.