HPLC-DAD测定大茶树和小茶树的西湖龙井茶中EGCG的含量

Quantitative Determination of EGCG of West Lake Longjing Tea from Big Tea Tree and Small Tea Tree by HPLC-DAD

[目的]采用HPLC-DAD对西湖龙井茶中的表没食子儿茶素没食子酸酯（EGCG）的含量变化进行分析.[方法]采用AgilentC18色谱柱（4.6 mm×150 mm,5μm）,流动相：甲醇：水（2％乙酸）15∶85;流速：1.0 ml/min;检测波长：276 nm;柱温：35℃.[结果]EGCG线性范围为0.152～3.040 μg,相关系数r为1,平均回收率97.61％,RSD为6.61％.[结论]该方法精密度、准确度良好,稳定性高,能简便、快速地测定大小茶树的西湖龙井茶中的EGCG含量.

[ Obiective ] HPLC-DAD is used to analyze the content of EGCG from West Lake Longjing tea in different periods. [ Method ] Agi- lent C18 （4.6 mm ×150 mm, 5 μm） is taken as the column, methanol solution and 2% acetic acid water solution （15：85, V/V） as the mo- bile phase, the velocity o f flow is 1.0 ml/min, the column temperature is 35 ℃, the inspect wave length is 276 nm. [ Result] The linear range of EGCG is 0. 152 - 3. 040 μg, r = 1, the average rate of recovery is 97.61% and the RSD is 6.61%. [ Conclusion ] The method was precise, accurate, highly stable, simple, convenient and rapid for the quantitative determination of EGCG.