AtNDPK2基因转化敖汉苜蓿及其耐盐性分析

Transgenic Alfalfa with AtNDPK2 and Salt Tolerance Analysis Plants

植物核苷二磷酸激酶（NDPKs），属于蛋白激酶家族，参与植物的多种生理生化过程，在植物响应非生物胁迫过程中发挥重要作用。NDPK2基因转化紫花苜蓿，对提高紫花苜蓿的耐逆性具有重要意义。试验以“敖汉”苜蓿下胚轴的再生系统为基础，通过农杆菌介导法将SWPA2驱动的AtNDPK2基因转入紫花苜蓿下胚轴，获得抗性植株。通过PCR初步检测证明该基因已经整合到“敖汉”苜蓿的基因组，阳性植株转化率为23．3％。不同浓度NaCl胁迫测定转基因植株的SOD、POD活性及质膜相对电导率、MDA含量，结果发现在0．4％～1．0％NaCl胁迫下转基因植株的SOD、POD酶活性显著高于对照，相对电导率、MDA含量显著的低于对照，进一步说明AtNDPK2基因的转入增强了“敖汉”苜蓿的耐盐性。

Nucleoside double phosphate kinase （NDPKs）belongs to the family of protein kinases,participating in physiological and biochemical processes in plants,playing an important role in the process of the abiotic stress.Transfor-mation of alfalfa NDPK2 gene would improve the resistance of alfalfa to play an important significance in reverse.This research was based on alfalfa hypocotyls regeneration system of “Aohan”,mediated by Agrobacterium tumefaciens to transform AtNDPK2 gene driven by SWPA2 into Alfalfa hypocotyls,get resistant plants.The result of PCR preliminary analysis showed that the gene had been integrated into the alfalfa genome of“Aohan”,positive plant transformation rate was 23.3%.The results on testing of transgenic plant SOD,POD activity and membrane relative conductivity and MDA under different concentrations of NaCl stress,showed that SOD,POD enzyme activity of transgenic plants under 0.4%~1 .0%NaCl stress was significantly higher than that of control;The relative conductivity and MDA content was signifi-cantly lower than that of control,further showed that AtNDPK2 cDNA increased the Salt-tolerance of“Aohan”alfalfa.