投加甲烷鬃菌对厌氧颗粒污泥培养的影响

Effect of Methanosaeta on anaerobic granular sludge formation

通过在中温条件下向EGSB(Expanded Granular Sludge Bed,膨胀颗粒污泥床)反应器中加入竹节状甲烷鬃菌6Ac,研究其对颗粒污泥形成的影响以及形成的颗粒污泥性质和反应器运行的情况.在(35±1)℃下,利用实验室相同规模的反应器,对照组(R1)接种厌氧絮状污泥,R2组接种厌氧絮状污泥的同时加入甲烷鬃菌,培养颗粒污泥.结果显示:当有机负荷(以COD计)逐步提高到11.1 kg m-3 d-1时,R2的COD去除率达到85.9%,高于R1的75.0%.运行到第46天(d 46)时两个反应器均可观测到颗粒污泥,R2中污泥粒径大于0.1 mm的颗粒污泥已经达到62.3%,d 28-46时,0.1-0.3mm颗粒的比例从10.0%增加到49.8%,对照组R1只从10%增加到33.3%,仍主要以絮状污泥为主.研究表明,颗粒污泥培养过程中投加甲烷鬃菌有利于促进颗粒污泥的形成、增殖与稳定,改善其沉降性能,提高有机物的去除效果,加快EGSB的启动.

In order to understand the effect of filamentous cells of Methanosaeta harundinacea 6Ac on granular sludge formation under the medium temperature in the EGSB reactor, as well as the properties of the granular sludge tbrmed and operation of the reactor, we cultivated granular sludge with two laboratory scale reactors under the constant temperature condition of 35 ±1℃. One reactor （R2） was inoculated anaerobic flocculent sludge with a bristle methane bacteria species; the other reactor （R1） was the control only inoculated with the anaerobic flocculent sludge. The results showed that when the organic load gradually increased to 11.1 kg m^-3 d^-1, the COD removal rate of R2 reached 85.9%, higher than that of RI （75.0%）. Granular sludge was founded in both reactors after 46 days, with 62.3% sludge particles greater than 0.1 mm in R2. During 28- 46 days, the percentage of sludge particles 0.1-0.3 mm increased from 10.0% to 49.8% in R2, but only from 10% to 33.3% in the control group R1, where flocculent sludge was mainly detected. This research showed that adding Methanosaeta could promote formation of granular sludge, enhance its proliferation and stability, improve precipitation of granular sludge and removal of organic matter, and accelerate start-up of EGSB.