丙肝病毒核心抗原的检测在诊断丙肝中的意义

Detection of HCV Core Antigen in the Diagnosis of Hepatitis C

目的通过分别检测丙肝病毒抗体(HCV-Ab)、丙肝病毒核心抗原(HCV-cAg)和定量检测HCVRNA,探究丙肝病毒核心抗原检测对于丙型肝炎诊断的意义。方法对36例丙肝抗体阳性患者和120例丙肝抗体阴性患者同时检测HCV-Ab、HCV-cAg和HCV-RNA。丙肝抗体检测采用间接ELISA法,丙肝核心抗原检测采用双抗体夹心ELISA法,丙肝HCV-RNA检测采用荧光PCR法定量检测。结果 36例丙肝抗体阳性组中,HCV-cAg阳性28例,HCV-RNA阳性25例。120例丙肝抗体阴性的门诊肝炎患者中HCV-cAg阳性有2例,HCV-RNA阳性有1例。结论 HCV-cAg检测可以缩短HCV抗体检测窗口期,特异性高,操作简便,可将二者联合起来,提高丙型肝炎病毒的检出率及准确度。

Objective Hepatitis C virus core antigen （HCV-cAg） was used to detect hepatitis C virus antibody （HCV-Ab）, and quantitative detection of HCV-RNA, to explore the signiifcance of the hepatitis C virus core antigen testing for hepatitis C diagnosis. Methods 36 cases of hepatitis C antibody-positive patients and 120 cases of hepatitis C antibody-negative patients with simultaneous detection of HCV-Ab, HCV-cAg HCV-of RNA. HCV antibodies were detected by indirect ELISA, HCV core antigen detection by double antibody sandwich ELISA HCV of HCV-RNA was detected by fluorescent PCR assay for quantification. Results 36 cases of hepatitis C antibody-positive group, cAg of HCV-positive in 28 cases, 25 cases of HCV-RNA positive. The 120 cases of hepatitis C antibody negative out-patient hepatitis patients HCV-cAg in positive cases, HCV-RNA positive 1 cases. Conclusions Detection of HCV-cAg can shorten the window period of HCV antibody detection, high speciifcity, easy to operate, can be combined together to improve the detection of hepatitis C virus and accuracy.