摘要
目的:建立能同时测定人血浆中拉米夫定(3TC)、齐多夫定(AZT)和奈韦拉平(NVP)浓度的方法。方法:血浆用乙腈沉淀处理后,以乙酸乙酯提取后进行高效液相色谱法测定。色谱柱为AgilentXDB.C-18.流动相为乙腈.水溶液(梯度洗脱),流速为O.8ml/min,检测波长为268nm,柱温为30℃,内标为甲硝唑。结果:3TC、AZT、NVP血药浓度分别在O.05~10μg/ml(r=0.9981)、0.05~10μg/ml(r=0.9990)、0.1~20μg/ml(r=0.9993)范围内线性关系良好,定量限分别为0.05、0.05、O.1gg/ml;平均方法回收率分别为96.36%~105.84%、98.73%~101.93%、98.78%~108.07%,日内、日间RSD〈8%。结论:本方法简便、快速、准确、灵敏,可用于同时使用3TC、AZT和NvP的艾滋病患者的临床治疗药物监测及药动学研究。
OBJECTIVE: To establish a sensitive and rapid method for the simultaneous determination of lamivudine (3TC), zid- ovudine (AZT) and nevirapine (NVP) in human plasma. METHODS: The plasma were extracted with ethyl acetate after precipitat- ed by acetonitrile. The determination was performed on Agilent XDB-C,8 with mobile phase consisted of acetonitrile-water (gradient elution) at the flow rate of 0.8 ml/min. The detection wavelength was 268 nm, and column temperature was 30 ℃. Metronidazole was used as the internal standard. RESULTS: The linear range of 3TC, AZP,NVP were 0.05-10 μg/ml (r=0.998 1), 0.05-10 μg/ml (r= 0.999 0), 0.1-20 μg/ml (r = 0.999 3), respectively; and the limits of quantitation was 0.05,0.05, 0.1 μg/ml respectively, the av- erage method recovery were 96.36%-105.84%, 98.73%-101.93%, 98.78%-108.07% ,respectively. The intra-day and inter-day RSD were all 〈8% o CONCLUSIONS: The method is simple and rapid, accurate, sensitive, and can be used for therapy drug monitor- ing and pharmacokinetic study of AIDS patients receiving 3TC, AZT and NVP simultaneously.
出处
《中国药房》
CAS
CSCD
2014年第18期1665-1668,共4页
China Pharmacy
基金
广东省医学科研基金立项课题(No.A2013523)
广州市医药卫生科技项目(No.201102A213157)