HPLC法同时测定人血浆中拉米夫定、齐多夫定和奈韦拉平的浓度

Simultaneous Determination of Lamivudine,Zidovudine and Nevirapine in Human Plasma by HPLC

目的：建立能同时测定人血浆中拉米夫定（3TC）、齐多夫定（AZT）和奈韦拉平（NVP）浓度的方法。方法：血浆用乙腈沉淀处理后，以乙酸乙酯提取后进行高效液相色谱法测定。色谱柱为AgilentXDB．C-18.流动相为乙腈．水溶液（梯度洗脱），流速为O．8ml／min，检测波长为268nm，柱温为30℃，内标为甲硝唑。结果：3TC、AZT、NVP血药浓度分别在O．05～10μg／ml（r=0．9981）、0．05～10μg／ml（r=0．9990）、0．1～20μg／ml（r=0．9993）范围内线性关系良好，定量限分别为0．05、0．05、O．1gg／ml；平均方法回收率分别为96．36％～105．84％、98．73％～101．93％、98．78％～108．07％，日内、日间RSD〈8％。结论：本方法简便、快速、准确、灵敏，可用于同时使用3TC、AZT和NvP的艾滋病患者的临床治疗药物监测及药动学研究。

OBJECTIVE： To establish a sensitive and rapid method for the simultaneous determination of lamivudine （3TC）, zid- ovudine （AZT） and nevirapine （NVP） in human plasma. METHODS： The plasma were extracted with ethyl acetate after precipitat- ed by acetonitrile. The determination was performed on Agilent XDB-C,8 with mobile phase consisted of acetonitrile-water （gradient elution） at the flow rate of 0.8 ml/min. The detection wavelength was 268 nm, and column temperature was 30 ℃. Metronidazole was used as the internal standard. RESULTS： The linear range of 3TC, AZP,NVP were 0.05-10 μg/ml （r=0.998 1）, 0.05-10 μg/ml （r= 0.999 0）, 0.1-20 μg/ml （r = 0.999 3）, respectively; and the limits of quantitation was 0.05,0.05, 0.1 μg/ml respectively, the av- erage method recovery were 96.36%-105.84%, 98.73%-101.93%, 98.78%-108.07% ,respectively. The intra-day and inter-day RSD were all 〈8% o CONCLUSIONS： The method is simple and rapid, accurate, sensitive, and can be used for therapy drug monitor- ing and pharmacokinetic study of AIDS patients receiving 3TC, AZT and NVP simultaneously.