抑郁模型大鼠海马齿状回神经干细胞增殖的研究

Proliferation Study of Hippocampal Neural Stem Cells in Dentate Gyrus of Depressed Model Rats

目的评价抑郁状态下海马神经再生能力。方法 20只Wistar大鼠随机分为对照组和抑郁症模型组(模型组),复制慢性不可预见性应激模型。观察大鼠体质量和行为学变化,放免法检测血浆促肾上腺皮质激素释放激素(CRH)、促肾上腺皮质激素(ACTH)和皮质酮(CORT)水平,光镜及电镜技术观察海马CA3神经元形态学变化,免疫组化和原位杂交技术检测海马齿状回巢蛋白(Nestin)及其mRNA表达。结果与对照组相比,模型组大鼠的体质量及其增长率下降,行为学得分减少,血浆CRH、ACTH和CORT显著升高,海马CA3神经元出现结构损伤,海马Nestin及其mRNA表达增加。结论抑郁状态下海马神经再生能力上升。

Objective To evaluate the hippocampal neurogenesis in depression. Methods A total of 20 Wistar rats were randomly divided into control and depression group （model group）. Chronic unpredicted stress model was established. Body mass weigh and behavioral scores of the rats were observed. Radioimmunoassay was used to measure plasma concentrations of corticotropin releasing hormone, adrenocorticotropic hormone and cortisone. Light and electron microscope were used to detect the morphological change of hippocanlpal CA3 neurons. Immunohistochemistry and in situ hybridization were used to detect both protein and mRNA expressions of Nestin in hippocampal dentate gyms. Results Compared with the con- trol group, rats in the model group, both body mass weigh and the related increase rate were markedly decreased, and the behavioral scores were re- duced significandy. In addition, neural damage was observed in hippocampal CA3 neurons, and the Nestin expressions in hippocampal dentate gyms were evidently increased. Conclusion Hippocampal neurogenesis was increased in depression.