陆英愈伤组织诱导及植株再生

Callus Induction and Plant Regeneration of Sambucus chinensis

目的:探索陆英愈伤组织和丛生芽诱导及组培苗生根的适宜条件,为陆英种苗工厂化生产及种质资源保存提供理论依据。方法:以叶片、叶柄、茎段和茎尖为外植体,比较不同外植体、不同植物激素对愈伤组织、丛生芽的诱导及生根培养的影响。结果:叶片、叶柄愈伤组织诱导的适宜培养基分别为MS+2,4-D 1.5 mg/L、MS+NAA 0.2 mg/L+KT 1.0 mg/L和MS+2,4-D 0.2 mg/L+KT 1.0 mg/L;丛生芽诱导与增殖适宜培养基为MS+NAA0.2 mg/L+KT 3.0 mg/L+GA32.0 mg/L;生根最佳培养基为1/2MS+NAA 0.3 mg/L,在此条件下,根发育良好,植株健壮。结论:叶片、叶柄在适宜的培养基中均可诱导出较好的愈伤组织;茎段、茎尖诱导丛生芽增殖系数高,完成植株再生。

Objective： To optimize the medium for callus and multiple shoot induction and cultural plantlets rooting of Sambucus chinensis,and to provide a basis for the development of the seedling of mass production and germplasm conservation. Methods： The plantlet leaves,petioles,stems and shoot tips were used as explants for the study of influences of different explants and different hormones on callus and multiple shoot induction,and cultural plantlets rooting. Results： The optimal medium for callus induction of leaves was MS ＋ 2,4-D 1. 5 mg / L; and optimum mediums for petiole were MS ＋ NAA 0. 2 mg / L ＋ KT 1. 0 mg / L and MS ＋ 2,4-D 0. 2 mg / L ＋ KT 1. 0 mg / L,MS ＋ NAA 0. 2 mg / L ＋ KT 3. 0 mg / L ＋ GA32. 0 mg / L medium was best for multiple shoot induction. And the best medium for tissue culture rooting was 1 /2 MS ＋ NAA 0. 3 mg / L. On the optimized medium,the roots were well-developed and the plants were healthier. Conclusion： Both the leaves and petioles can be well induced to callus on suitable medium,the stems and shoot tips are the best materials for multiple shoot induction and plant regeneration.