不同生长期毛郁金挥发性成分的GC-MS分析

GC-MS Analysis of Essential Oil from Curcuma aromatica Rhizome of Different Growth Periods

目的:研究毛郁金根茎不同生长期挥发性化学成分,为毛郁金的合理栽培及质量标准制订提供参考。方法:采用水蒸气蒸馏法提取毛郁金挥发油,应用GC毛细管柱进行分离,质谱检测,面积归一化法测定其相对含量。结果:各生长期毛郁金根茎的主要挥发性成分基本相同,其中总含量最高的为姜黄二酮,横县野生品含量最高,达16.35%,南宁明阳农场栽培一年生毛郁金含量也较高,为15.81%;其次含量较高的为桉油精,横县栽培二年生毛郁金含量高达15.40%,横县野生品含量为14.59%;其他含量较高的有β-榄香烯、β-石竹烯、丁香酚和吉马酮等萜类化合物。结论:不同生长期的毛郁金挥发性成分基本相同,但主要成分含量有别,此结果为推广毛郁金种植生产提供了依据。

Objective： To analyze the essential oil from the rhizome of Curcuma aromatica of different growth periods,and to provide the scientific reference for reasonable cultivation and quality control of this plant. Methods： The essential oil was extracted by hydrodistillation and analyzed with GC-MS. The relative contents were determined with area normalization method. Results： The main volatile constituents in the rhizome of Curcuma aromatica were basically the same. Among these volatile constituents,curdione was the major. The relative content of curdione was 16. 35% in the rhizome of wild plant in Hengxian county,and 15. 81% in the rhizome of one-year-old plant in Mingyang farm,Nanning city. The relative content of eucalyptol in the 2-year-old cultivated rhizome in Hengxian county was 15. 40%,and 14. 59% in the rhizome of wild plant in Hengxian county. β-Elemene,β-caryophyllene,eugenol and germacrone were also the main constituents in the rhizome essential oil. Conclusion： Volatile constituents in the rhizome of Curcuma aromatica are similar to each other,but the relative content of each component is different. This result can provide the scientific foundation for the cultivation of Curcuma aromatica.