生地黄免疫抑制活性部位HPLC指纹图谱研究

Fingerprint Research on Immunosuppressive Fraction of Rehmanniae Radix by HPLC

目的:研究河南省道地药材生地黄免疫抑制活性部位HPLC指纹图谱。方法:采用高效液相色谱法,迪马Platisil-C18柱(250 mm×4.6 mm,5μm),乙腈-0.1%磷酸梯度洗脱,检测波长为210 nm,流速为1.0 mL/min,柱温为30℃。结果:对10批生地黄免疫抑制活性部位的指纹图谱进行了相似度比较,标定共有峰18个,其中5个色谱峰成分已指认,相似度在0.9以上。结论:建立了生地黄免疫抑制活性部位HPLC指纹图谱,为地黄进一步的药效学研究奠定了基础。

Objective： To study the HPLC fingerprint of immunosuppressive fraction of Rehmanniae Radix,the genuine medicinal materials in Henan province. Methods： By HPLC method,Dikma Platisil-C18column（ 250 mm × 4. 6 mm,5 μm） was used with acetonitrile-0. 1% phosphoric acid as mobile phase by gradient elution. The detection wavelength was 210 nm,flow rate was 1. 0 mL / min and column temperature was set at 30 ℃. Results： Compared the 10 batches sample of the fingerprint similarity,18 common peaks were marked and five peaks were identified. The similarities were more than 0. 9. Conclusion： HPLC fingerprint of immunosuppressive fraction of Rehmanniae Radix is established,which lays the foundation for further pharmacodynamics research on Rehmanniae Radix.