响应面法优化发酵藤茶黄酮和多糖的综合提取工艺

Response Surface Analysis for Optimization of Comprehensive Extraction of Flavonoid and Polysaccharide in Fermented Ampelopsis grossedentata

目的:探讨发酵藤茶黄酮和多糖的综合提取工艺。方法:根据单因素试验的结果,采用响应面分析法优化藤茶黄酮和多糖的综合提取工艺。结果:最佳提取工艺条件为:提取温度96℃,时间1.6 h,料液比1∶21。在此工艺条件下,总黄酮提取得率为22.94%,多糖提取得率为2.13%。结论:为藤茶黄酮和多糖的综合提取工艺提供了参考,有利于藤茶资源的综合开发利用。

Objective： To explore the comprehensive extraction conditions of flavonoid and polysaccharide in fermented Ampelopsis grossedentata. Methods： On the basis of single factor experiments,response surface analysis（ RSA） was used to optimize the extraction of flavonoid and polysaccharide in fermented Ampelopsis grossedentata. Results： The optimal conditions were as follows： temperature 96 ℃,time 1. 6 h,solid-liquid ratio 1∶ 21. Under these conditions,the total flavonoids extraction yield was 22. 94%,the polysaccharide extraction yield was 2. 13%. Conclusion： This study provides a reference for the comprehensive extraction of flavonoid and polysaccharide in fermented Ampelopsis grossedentata,and lays a foundation for the comprehensive development and utilization of Ampelopsis grossedentata.