仪器法快速检测鲜乳微生物的可靠性研究

Reliability of Rapid Detection Fresh Milk Microorganism by Insrument Method

本文通过乳制品微生物活性快速检测仪对鲜乳中微生物酶活性的测定,快速检测鲜乳中微生物数量的变化,判定鲜乳品质的优劣。试验结果表明:仪器法可以在30min内完成一个鲜乳样品的微生物含量的检测。利用鲜乳微生物快速检测仪和国标平板菌落计数法分别检测大肠杆菌、蜡样芽胞杆菌、变形杆菌、灰绿曲霉、黑曲霉、球拟酵母菌、金黄色葡萄球菌、伤寒沙门氏菌和志贺氏菌等主要危害鲜乳品质的菌落数量,两种方法的测定结果相关系数均在0.98以上,这表明两种方法间有很好的线性相关性,同样用两种方法监测鲜乳贮藏过程中的微生物活动情况,其检测结果 R2=0.991,属于显著相关。

Method of fast detecting fresh milk microorganism was investigated by determining the enzyme activity with quick microorganism detector, which could monitor quality of fresh milk. Experimental resuhs showed that detection of microbial content instrument method eould be accomplished with a fresh milk samples in 30 minutes. Detect Escherichia coli, Bacwillus cereus, Bacillus Proteus, Aspergillus glaucus, Kuroma, Torulopsis, Staphylococcus aureus, Salraonella typhi, Shigella and other major hazards of fresh milk quality by the number of colonies using fresh milk microbial rapid detection instrument and standard plate count method. Determination results of two methods of correlation coeffieient was above 0.98, indicating that two methods had good linear correlation. Microbial activity of fresh milk two methods during storage, the R^2=0.991, which belonged to significant correlation.