氧化低密度脂蛋白受体在人血管内皮细胞上的表达及其调控

Indentification of oxidized LDL receptor in umbilical vein and regulation of its gene expression by oxidized

采用放射配基结合及竞争抑制实验和反转录 聚合酶链反应检测人脐静脉内皮细胞 (HUVECs)上血凝素样的氧化低密度脂蛋白受体 (LOX1)的表达 ,表明HUVECs存在LOX1的基因表达和高亲和力位点Bmax (5 4 5±2 0 3)ng/ 10 6cell,Kd (2 0 1± 0 6 )× 10 -8mol/L ,氧化低密度脂蛋白对其自身受体LOX1的基因表达为正反馈调节 ,应用LOX1抑制剂可抑制此作用。

Human umbilical vein endothelial cells(HUVECs) express LOX1 mRNA and possess high affinity binding sites for LOX1 by radioligand binding assays and reverse transcription polymerase chain reaction(RT PCR). Oxidized LDL upregulated LOX1 mRNA expression in a concentration dependent way, polyinosinic acid, the inhibitor of LOX1 receptor, inhibited this effects.