利用PCR构建多拷贝HIV-1 TAR序列的串联体

Amplification of the multimerized HIV-1 TAR by PCR

建立“自身引物—模板”PCR方法 ,并应用此方法成功地扩增了多拷贝HIV 1TAR片段的同向串联体。此方法简便 ,省力 ,可用于多拷贝基因探针、多拷贝反义基因片段、多拷贝抗原表位的扩增和克隆。

Primer pair was designed as self template during the amplification by PCR. It’s an easy way to construct a series of multi copy genes. This method can be available for the amplification of DNA probes and the development of vaccines. It’s considered as a convenient and simple method for the cloning of multi copy genes.