Periostin对人牙周膜细胞增殖和碱性磷酸酶活性的影响

The effects of mouse recombinant Periostin on proliferation and ALP activity of PDL cells.

目的 :研究小鼠重组Periostin对牙周膜细胞 (PDL细胞 )增殖及碱性磷酸酶 (ALP)活性的影响。方法 :采用MTT比色试验及酶动力学方法 ,测定PDL细胞的增殖和ALP活性。结果 :Periostin仅在浓度为 40 μg/ml时 ,可显著促进PDL细胞的增殖 (P <0 .0 1) ,其余浓度则无作用 ,而其浓度在 10 μg/ml～ 80 μg/ml范围中时 ,均可显著提高PDL细胞ALP活性水平 (P <0 .0 5 )。结论 :小鼠重组Periostin对PDL细胞的增殖及ALP活性均有促进作用 ,但其在这两方面的作用存在差异 。

Objective:To investigate the effects of mouse recombinant Periostin on proliferation and ALP activity of periodontal ligament cells (PDL cells).Methods:MTT method and enzyme dynamics method were used to observe the ability of proliferation and ALP expression of PDL cells.Results:Significant proliferation of PDL cells could be observed on Periostin at dose 40ug/ml ( P <0.01) and the ALP activity was increased at dose10μg/ml～80μg/ml( P <0.05).Conclusion:Periostin can promote the proliferation and ALP activity of PDL cells,but the effects need further studies. [