复发性口疮患者静止期与溃疡期T抑制细胞功能比较研究

The Comparison of T Suppressor Function at Remission and Ulcerative Stages of Patients with RAU.

目的 :本研究旨在比较RAU患者溃疡期和静止期外周血淋巴细胞抑制活性 ,探索T抑制细胞在RAU发病学中的作用。方法 :应用刀豆碱 (ConA)激活产生的淋巴细胞抑制检测方法探测了 12位RAU患者溃疡期和静止期外周血淋巴细胞抑制活性。结果 :溃疡期患者的T淋巴细胞抑制率明显低于正常组 (P <0 .0 5 ) ;而患者静止期的抑制率与正常组无显著性差异 (P >0 .0 5 )。结论 :RAU患者外周血T抑制细胞功能在溃疡期降低 。

Objective:The aim of the current study was to investigate the ability of peripheral blood cells to mount a satisfactory level of suppressor activity.Methods:12 RAU patients at active and remission phases and 15 healthy persons were selected in this project.All subjects were taken 40 ml of venous blood at the same time on each day of experimentation.PBMC were isolated by density gradient centrifugation and divided into three fractions (AE, NE and SR).The suppressor activity at different concentration of Con A was measured by a concanavalin A generated suppressor assay system.Results:Samples obtained from 12 RAU patients and a control group showed a depression in suppressor activity in RAU patients that is disease phase related with return to normal levels in remission.Conclusion:These findings suggest that alterations in suppressor activity are not a primary event in the initiation of RAU lesions and do not have any controlling influence on the progress of individual lesions. [