塞隆骨原动物高原鼢鼠核基因18SrRNA序列测定与分析

Nuclear Ribosomal RNA Small Subunit (18S rRNA) Nucleotide Sequencing and Characterization of Sailonggu(Whole Bone of Myospalax baileyi Thomas)

目的 :测定仓鼠科动物高原鼢鼠Myospalaxbaileyi的核rDNA基因序列 ,为塞隆骨正品基原检定提供分子依据。方法 :采用PCR直接测序技术测定高原鼢鼠 18SrRNA基因核苷酸序列并作序列特征分析。结果 :高原鼢鼠的 18SrRNA序列长度为 185 1bp。根据排序比较 ,高原鼢鼠与 2种鼠科动物间的DNA序列同源性为 72 .0 4%～ 72 .18%。结论 :通过基因序列分析 ,DNA测序技术可成为塞隆骨正品基原检定的准确有效手段。

Objective: Sequencing the nuclear ribosomal RNA small subunit (18S rRNA) gene of Myospalax baileyi (Cricetidae) to develop an ultimate and definitive means for origin identification of genuine Sailonggu. Methods: The total DNA was prepared from dried tail tissues. The nuclear 18S rRNA gene region was amplified by PCR using a consensus primer set and its nucleotide sequence was determined by PCR direct sequencing. The characteristic analysis of 18S rRNA sequences was generated usin software program Genetyx SV/R Version 10.1. Results: The entire 18S rRNA gene region of M. baileyi spanned 1851 bp in length. Although multiple alignment of sequence indicates that there are only lower homology (72.04%～72.18%)comparing with its two alias Mus musculus (GenBank Accession number X00686)and Rattus norvegicus (M11188)(Muridae), their highly conservative domain is located in 1020～1509 nt. There are many variable sites from upstream of 5' end, which coud provide a novel information for molecular recognition of Sailonggu. Conclusion:DNA sequencing could be a useful and reliable tool in the origin identification of genuine Sailonggu. [