六味地黄汤对佐剂性关节炎大鼠脾脏细胞表达细胞因子的影响

Effect of Liuwei Dihuang Decoction, on the Cytokine Expression in Splenocytes in AA Rats

目的 :观察六味地黄汤 (LW )对佐剂性关节炎 (AA)大鼠脾脏辅助T细胞 (Th)的作用 ,并与免疫抑制剂环磷酰胺 (Cy)、环孢素A(CsA)进行比较。 方法 :运用定量PCR技术 ,检测AA大鼠脾脏T淋巴细胞因子IFN γ ,IL 2 ,IL 4和IL 10的mRNA的表达水平。结果 :AA大鼠脾淋巴细胞IL 2mRNA表达水平与对照组相比有增高趋势 ;IFN γ ,IL 4和IL 10mRNA的表达水平则明显降低。口服LW对AA大鼠脾细胞的表达水平则具有明显提高作用 ,与Cy和CsA相比具有明显不同的特点。 结论 :LW可能对AA大鼠脾脏表达的细胞因子水平有调节作用 ,提示LW可纠正Th1/Th2亚群功能的平衡紊乱。

Objective: The effect of Liuwei Dihuang decoction (LW) on the function of splenic T helper cells (Th) was studied and compared with those of the immune inhibitors such as cyclophosphamide (Cy) and cyclosporin A (CsA) in AA rats. Methods: The expression of mRNA for IFN γ,IL 2, IL 4 and IL 10 in splenic T Lymphocytes of adjuvant arthritis (AA) rats was evaluated using reverse transcription polymerase chain reaction (RT PCR) technique. Results: mRNA expression level of IL 2 had a tendency to elevate, but the mRNA expression levels of IFN γ,IL 4 and IL 10 were significantly decreased in AA rats in comparison with control groups. LW treatment significantly inhibited the mRNA expression of IL 2 and promoted the expression of IFN γ,IL 4 and IL 10 in splenocytes of AA rats, with an obvious characteristic as compared with that of Cy or CsA. Conclusion: LW can correct the imdifferent balance of the functions of splenocyte Th1/Th2 in AA rats. [