端粒酶hTERT与宫颈癌SiHa细胞增殖及侵袭能力研究

Telomerase SiHa HTERT and cervical cancer cell proliferation and invasion ability of study

目的本文主要对端粒酶hTERT与宫颈癌SiHa细胞增殖及侵袭能力进行研究。方法应用免疫组化SABC法检测hTERT及Bcl-2在80例宫颈鳞癌及20例正常宫颈组织中的表达。采用MTT法测定015对Siha细胞增殖的抑制作用。结果在正常宫颈组织和宫颈癌组织中,hTERT表达的阳性率与表达阳性率,对比差异显著,具有统计意义(P<0.05)。MTT法检测显示Ori有利于抑制SiHa细胞的生长,且与浓度和时间具有正相关的联系。结论相对于正常的正常宫颈组织,hTERT、Bcl-2蛋白在宫颈鳞癌组织中阳性表达率比较高。Ori有利于抑制SiHa细胞增殖及侵袭能力。

Objective： This article mainly discusses the telomerase SiHa hTERT and cervical cancer cell proliferation and invasive ability were studied. Methods： The immunohistochemical SABC method to detect hTERT and Bcl- 2 in 80 cases of cervical squamous carcinoma and 20 cases of normal cervical tissue. Determined by MTT method is used to measure 015 pairs of Siha cell proliferation inhibition. Results： In normal cervical tissues and cervical cancer tissues,the positive rate and expression of hTERT expression positive rate,compared with significant difference,statistically significant（ P 0. 05）. Determined by MTT method to detect show Ori is beneficial to inhibit the growth of SiHa cells,and they have positive correlation with concentration and time of contact. Conclusion： Compared with normal normal cervical tissue,hTERT,Bcl- 2 protein in cervical squamous carcinoma tissue in the positive expression rate is quite high. Ori inhibition of SiHa cells proliferation and invasive ability.