摘要
目的研究子痫前期患者蜕膜组织中Th17和Treg细胞转录因子的表达水平,并探讨其在子痫前期发病机制中的作用。方法选取重度子痫前期患者蜕膜组织32例作为实验组,健康妊娠妇女蜕膜组织32例作为对照组,采用RTPCR方法,检测各组研究对象中RORγt mRNA和Foxp3 mRNA的表达水平。结果 1.子痫前期重度组蜕膜组织中RORγt mRNA水平明显高于对照组(P<0.01),而Foxp3 mRNA水平明显低于对照组(P<0.01)。2.RORγt mRNA/Foxp3mRNA在子痫前期重度组的比值高于对照组(P<0.05)。结论子痫前期患者蜕膜组织中存在明显的Th17/Treg细胞免疫失衡,可能是导致子痫前期发生的重要原因。
Objective： To investigate the expression levels of Th17 cells and Treg cells＇ s transcriptions factors and to explore their role in pathogenesis in patient＇s deciduas with pre- eclampsia. Methods： Select preeclampsia（ severe） patients decidua 32 cases as the experimental group,32 women with normal pregnancy decidua as a control group,using RT- PCR method to detect the maternal decidua RORγt mRNA and Foxp3 mRNA levels expression. Results： 1. There was significant increase in Th17 cell and RORγt mRNA level in patients deciduas with pre- eclampsia（ P 0. 01）. Meanwhile,there was significant reduction in Treg cell frequency and Foxp3 mRNA level in patients deciduas with pre- eclampsia（ P 0. 01）. 2. The ratio of RORγt mRNA / Foxp3 mRNA in deciduas of patients with pre- eclampsia is higher than normal pregnancy group（ P 0. 05）. Conclusion： The deciduas Th17 / Treg imbalance may plays an important role in pre- eclampsia.
出处
《中国优生与遗传杂志》
2014年第3期28-29,35,共3页
Chinese Journal of Birth Health & Heredity
