荧光定量PCR在几种性传播疾病检测中的临床结果分析

Analysis of the clinical results of fluorescence quantitative PCR in detection of sexually transmitted diseases

目的探讨性病检测中荧光定量PCR(FQ-PCR)法检测效果研究。方法选择行性传播病检测的患者190例和健康体检者40例,采用FQ-PCR方法,将其检测结果与其他方法检测结果进行比较,就临床资料行回顾性分析。结果所有标本均采用培养法、常规PCR和FQ-PCR进行UU、CT、NG检测,健康体检者40例均为阴性。患者190例3种检测结果分别为:培养法:UU 77例、CT 54例、NG79例;常规PCR:UU 78例、CT 58例、NG81例;FQ-PCR:UU 87例、CT 67例、NG91例。FQ-PCR优于培养法和常规PCR检测法,差异有统计学意义(P<0.05)。FQ-PCR法对UU、CT、NG的检测特异性、灵敏度、阳性和阴性预期值均显著高于培养法和常规PCR检测法,差异有统计学意义(P<0.05)。结论与培养法和常规PCR法比较,FQ-PCR法克服了其缺点,在性病检测中可达到较低污染、高精确性、特异性、灵敏度的特点,在实验室诊断中具有重要的价值。

Objective ： To investigate the fluorescence quantitative PCR in detection of sexually transmitted diseases （ FQ - PCR） method was used to detect the effect of. Methods： 40 cases of 190 cases of patients with venereal disease detection and healthy con- trols, using FQ - PCR method, the detection results of the detection results are compared with the other methods, the clinical data were retrospectively analyzed. Results ： All specimens by culture method, the conventional PCR and FQ - PCR were UU, CT, NG, and 40 healthy cases were negative. In 190 patients with three detection results were： Culture： UU 77 cases, CT 54 cases, NG79 cases; con- ventional PCR： UU 78 eases, CT 58 eases, FQ-PCR： UU 87 cases, NG8I cases; CT 67 cases, NG91 eases. FQ- PCR is better than that of culture for the detection method and the conventional PCR, the difference was statistically significant （ P 〈 O. 05 ）. The FQ - PCR method for UU, CT, NG detection sensitivity, specificity, positive and negative predictive values were significantly higher than that of culture for the detection method and the conventional PCR, the difference was statistically significant （ P 〈 O. 05 ）. Conclu- sions ： Compared with the culture method and the conventional PCR method, FQ - PCR method to overcome its shortcomings, features in STD detection can achieve low pollution, high accuracy, specificity, sensitivity, has important value in laboratory diagnosis.